Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats

Akio Itoh, Atsumi Nitta, Masayuki Nadai, Kyoko Nishimura, Mitsuhiko Hirose, Takaaki Hasegawa, Toshitaka Nabeshima

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Accumulations of β-amyloid protein are characteristic and diagnostic features of the brain of Alzheimer's disease patients; however, the physiological role of this protein in CNS is unknown. We have previously reported that continuous infusion of β-amyloid protein into rat cerebral ventricle impairs learning ability and decreases choline acetyltransferase activity, a marker enzyme of cholinergic neuron. In this study, the effects of β-amyloid protein infusion on the release of neurotransmitters in cholinergic and dopaminergic neuronal systems were investigated by using an in vivo brain microdialysis method. Nicotine-stimulated release of acetylcholine and dopamine in these animals was significantly lower than that in vehicle-infused rats. Further, dopamine release induced by high-K stimulation was decreased in β-amyloid protein-infused rats compared with vehicle-infused rats. These results suggest that the release of the two transmitters, acetylcholine and dopamine, was decreased by β-amyloid protein and that learning deficits observed in the β-amyloid protein-infused rats are partly due to the impairment of neurotransmitter release. Furthermore, continuous infusion of β-amyloid protein may be a useful method to produce the animal model of Alzheimer's disease.

Original languageEnglish
Pages (from-to)1113-1117
Number of pages5
JournalJournal of Neurochemistry
Volume66
Issue number3
Publication statusPublished - 01-03-1996
Externally publishedYes

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Amyloidogenic Proteins
Cholinergic Agents
Rats
Dopamine
Acetylcholine
Neurotransmitter Agents
Brain
Alzheimer Disease
Animals
Learning
Cerebral Ventricles
Cholinergic Neurons
Aptitude
Choline O-Acetyltransferase
Microdialysis
Brain Diseases
Nicotine
Neurons
Transmitters
Animal Models

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Itoh, A., Nitta, A., Nadai, M., Nishimura, K., Hirose, M., Hasegawa, T., & Nabeshima, T. (1996). Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats. Journal of Neurochemistry, 66(3), 1113-1117.
Itoh, Akio ; Nitta, Atsumi ; Nadai, Masayuki ; Nishimura, Kyoko ; Hirose, Mitsuhiko ; Hasegawa, Takaaki ; Nabeshima, Toshitaka. / Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 3. pp. 1113-1117.
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Itoh, A, Nitta, A, Nadai, M, Nishimura, K, Hirose, M, Hasegawa, T & Nabeshima, T 1996, 'Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats', Journal of Neurochemistry, vol. 66, no. 3, pp. 1113-1117.

Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats. / Itoh, Akio; Nitta, Atsumi; Nadai, Masayuki; Nishimura, Kyoko; Hirose, Mitsuhiko; Hasegawa, Takaaki; Nabeshima, Toshitaka.

In: Journal of Neurochemistry, Vol. 66, No. 3, 01.03.1996, p. 1113-1117.

Research output: Contribution to journalArticle

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Itoh A, Nitta A, Nadai M, Nishimura K, Hirose M, Hasegawa T et al. Dysfunction of cholinergic and dopaminergic neuronal systems in β-amyloid protein-infused rats. Journal of Neurochemistry. 1996 Mar 1;66(3):1113-1117.