E-selectin expression in a murine model of chronic colitis

Shigeyuki Kawachi, Zenichi Morise, Elaine Conner, F. Stephen Laroux, Laura Gray, John Fuseler, Matthew B. Grisham

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9 Citations (Scopus)

Abstract

The objective of this study was to quantify E-selectin surface expression in the colon as well as other tissues in a CD4+ T-cell model of chronic colitis in mice using the newly developed dual radiolabel monoclonal antibody technique. Male SCID mice were reconstituted with either 5 x 106 CD4+ CD45RB(low) or CD45RB(high) T-cells isolated from normal CB-17 donor mouse spleens and subsequently monitored for clinical signs of colitis. We found that animals injected with CD45RB(high) but not CD45RB(low) T-cells nor PBS developed colitis at 6-8 weeks following reconstitution as assessed by loss of body weight, development of loose stools and/or diarrhea, and histopathology. Concurrent with the onset of distal bowel inflammation was enhanced expression of E-selectin compared to SCID mice injected with PBS or reconstituted with CD45RB(low) T-cells, both of which did not develop colitis. We also observed significant increases in E-selectin expression in cecum, small intestine, mesentery, and liver of colitic mice. Our data confirm that reconstitution of SCID mice with CD45RB(high) but not CD45RB(low) T-cells induces chronic colitis and demonstrate that this chronic colitis is associated with enhanced expression of an endothelial cell-specific adhesion molecule. Furthermore, our studies demonstrate that reconstitution of SCID mice with CD45RB(high) T-cells enhances E-selectin expression in a variety of tissues distant from the site of active inflammation. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume268
Issue number2
DOIs
Publication statusPublished - 16-02-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kawachi, S., Morise, Z., Conner, E., Laroux, F. S., Gray, L., Fuseler, J., & Grisham, M. B. (2000). E-selectin expression in a murine model of chronic colitis. Biochemical and Biophysical Research Communications, 268(2), 547-552. https://doi.org/10.1006/bbrc.2000.2175