E-selectin targeting to visualize tumors in vivo

Masahiko Hirai, Yoshie Hiramatsu, Shinki Iwashita, Takayuki Otani, Ling Chen, Yue Guang Li, Masashi Okada, Kazunori Oie, Koich Igarashi, Hideaki Wakita, Masaharu Seno

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-α in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalContrast Media and Molecular Imaging
Volume5
Issue number2
DOIs
Publication statusPublished - 01-03-2010

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E-Selectin
Liposomes
Neoplasms
Angiogenesis Inducing Agents
Ehrlich Tumor Carcinoma
Antibodies
Optical Imaging
Human Umbilical Vein Endothelial Cells
Fluorescent Dyes
Endothelial Cells
Monoclonal Antibodies
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Hirai, M., Hiramatsu, Y., Iwashita, S., Otani, T., Chen, L., Li, Y. G., ... Seno, M. (2010). E-selectin targeting to visualize tumors in vivo. Contrast Media and Molecular Imaging, 5(2), 70-77. https://doi.org/10.1002/cmmi.367
Hirai, Masahiko ; Hiramatsu, Yoshie ; Iwashita, Shinki ; Otani, Takayuki ; Chen, Ling ; Li, Yue Guang ; Okada, Masashi ; Oie, Kazunori ; Igarashi, Koich ; Wakita, Hideaki ; Seno, Masaharu. / E-selectin targeting to visualize tumors in vivo. In: Contrast Media and Molecular Imaging. 2010 ; Vol. 5, No. 2. pp. 70-77.
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Hirai, M, Hiramatsu, Y, Iwashita, S, Otani, T, Chen, L, Li, YG, Okada, M, Oie, K, Igarashi, K, Wakita, H & Seno, M 2010, 'E-selectin targeting to visualize tumors in vivo', Contrast Media and Molecular Imaging, vol. 5, no. 2, pp. 70-77. https://doi.org/10.1002/cmmi.367

E-selectin targeting to visualize tumors in vivo. / Hirai, Masahiko; Hiramatsu, Yoshie; Iwashita, Shinki; Otani, Takayuki; Chen, Ling; Li, Yue Guang; Okada, Masashi; Oie, Kazunori; Igarashi, Koich; Wakita, Hideaki; Seno, Masaharu.

In: Contrast Media and Molecular Imaging, Vol. 5, No. 2, 01.03.2010, p. 70-77.

Research output: Contribution to journalArticle

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Hirai M, Hiramatsu Y, Iwashita S, Otani T, Chen L, Li YG et al. E-selectin targeting to visualize tumors in vivo. Contrast Media and Molecular Imaging. 2010 Mar 1;5(2):70-77. https://doi.org/10.1002/cmmi.367