Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia

Yuyan Zhu, Kuniaki Saito, Yuki Murakami, Masahide Asano, Yoichiro Iwakura, Mitsuru Seishima

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

There is convincing evidence that cytokines are involved in the inflammatory response following cerebral ischemia, but the interactions among the pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in the early stage of ischemic reperfusion are not yet completely understood. In this study, we examined the early mRNA expressions of pro-inflammatory cytokines in the ischemic hippocampus after 30 min of bilateral common carotid artery occlusion in C57BL/6J wild-type (WT) and TNF-α, IL-1α/β or IL-6 gene knockout (KO) mice utilizing real-time polymerase chain reaction. The mRNA expressions of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were significantly induced in ischemic WT mice compared with in the sham-operated mice. These increases peaked at 3 to 24 h for TNF-α, at 12 h for IL-1β, and at 6 to 24 h for IL-6 after ischemia. The pattern of temporal expression of the cytokine mRNAs in ischemic gene KO mice, however, differed from that in WT mice. The TNF-α mRNA expression showed a similar temporal expression pattern in IL-6 KO mice compared to in WT mice following ischemic reperfusion, and the levels at all time points were lower than in WT mice. The IL-1β mRNA level was very low in ischemic TNF-α KO mice and IL-6 KO mice in spite of a small peak observed in both at 24 h. The IL-6 mRNA level was significantly upregulated at all time points in both ischemic WT and TNF-α KO mice; however, the peak was delayed by 12-h in IL-1α/β KO mice. In conclusion, the present study indicates that the rapid increases in cytokine levels are interdependent, interactive, and possibly modulate each other in the mouse hippocampus after transient global ischemia.

Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalNeuroscience Letters
Volume393
Issue number2-3
DOIs
Publication statusPublished - 30-01-2006
Externally publishedYes

Fingerprint

Knockout Mice
Interleukin-1
Hippocampus
Ischemia
Tumor Necrosis Factor-alpha
Interleukin-6
Cytokines
Messenger RNA
Gene Knockout Techniques
Reperfusion
Common Carotid Artery
Brain Ischemia
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Zhu, Yuyan ; Saito, Kuniaki ; Murakami, Yuki ; Asano, Masahide ; Iwakura, Yoichiro ; Seishima, Mitsuru. / Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia. In: Neuroscience Letters. 2006 ; Vol. 393, No. 2-3. pp. 122-126.
@article{ba96687127fe445b828a2cd7ef21107c,
title = "Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia",
abstract = "There is convincing evidence that cytokines are involved in the inflammatory response following cerebral ischemia, but the interactions among the pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in the early stage of ischemic reperfusion are not yet completely understood. In this study, we examined the early mRNA expressions of pro-inflammatory cytokines in the ischemic hippocampus after 30 min of bilateral common carotid artery occlusion in C57BL/6J wild-type (WT) and TNF-α, IL-1α/β or IL-6 gene knockout (KO) mice utilizing real-time polymerase chain reaction. The mRNA expressions of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were significantly induced in ischemic WT mice compared with in the sham-operated mice. These increases peaked at 3 to 24 h for TNF-α, at 12 h for IL-1β, and at 6 to 24 h for IL-6 after ischemia. The pattern of temporal expression of the cytokine mRNAs in ischemic gene KO mice, however, differed from that in WT mice. The TNF-α mRNA expression showed a similar temporal expression pattern in IL-6 KO mice compared to in WT mice following ischemic reperfusion, and the levels at all time points were lower than in WT mice. The IL-1β mRNA level was very low in ischemic TNF-α KO mice and IL-6 KO mice in spite of a small peak observed in both at 24 h. The IL-6 mRNA level was significantly upregulated at all time points in both ischemic WT and TNF-α KO mice; however, the peak was delayed by 12-h in IL-1α/β KO mice. In conclusion, the present study indicates that the rapid increases in cytokine levels are interdependent, interactive, and possibly modulate each other in the mouse hippocampus after transient global ischemia.",
author = "Yuyan Zhu and Kuniaki Saito and Yuki Murakami and Masahide Asano and Yoichiro Iwakura and Mitsuru Seishima",
year = "2006",
month = "1",
day = "30",
doi = "10.1016/j.neulet.2005.08.072",
language = "English",
volume = "393",
pages = "122--126",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia. / Zhu, Yuyan; Saito, Kuniaki; Murakami, Yuki; Asano, Masahide; Iwakura, Yoichiro; Seishima, Mitsuru.

In: Neuroscience Letters, Vol. 393, No. 2-3, 30.01.2006, p. 122-126.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Early increase in mRNA levels of pro-inflammatory cytokines and their interactions in the mouse hippocampus after transient global ischemia

AU - Zhu, Yuyan

AU - Saito, Kuniaki

AU - Murakami, Yuki

AU - Asano, Masahide

AU - Iwakura, Yoichiro

AU - Seishima, Mitsuru

PY - 2006/1/30

Y1 - 2006/1/30

N2 - There is convincing evidence that cytokines are involved in the inflammatory response following cerebral ischemia, but the interactions among the pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in the early stage of ischemic reperfusion are not yet completely understood. In this study, we examined the early mRNA expressions of pro-inflammatory cytokines in the ischemic hippocampus after 30 min of bilateral common carotid artery occlusion in C57BL/6J wild-type (WT) and TNF-α, IL-1α/β or IL-6 gene knockout (KO) mice utilizing real-time polymerase chain reaction. The mRNA expressions of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were significantly induced in ischemic WT mice compared with in the sham-operated mice. These increases peaked at 3 to 24 h for TNF-α, at 12 h for IL-1β, and at 6 to 24 h for IL-6 after ischemia. The pattern of temporal expression of the cytokine mRNAs in ischemic gene KO mice, however, differed from that in WT mice. The TNF-α mRNA expression showed a similar temporal expression pattern in IL-6 KO mice compared to in WT mice following ischemic reperfusion, and the levels at all time points were lower than in WT mice. The IL-1β mRNA level was very low in ischemic TNF-α KO mice and IL-6 KO mice in spite of a small peak observed in both at 24 h. The IL-6 mRNA level was significantly upregulated at all time points in both ischemic WT and TNF-α KO mice; however, the peak was delayed by 12-h in IL-1α/β KO mice. In conclusion, the present study indicates that the rapid increases in cytokine levels are interdependent, interactive, and possibly modulate each other in the mouse hippocampus after transient global ischemia.

AB - There is convincing evidence that cytokines are involved in the inflammatory response following cerebral ischemia, but the interactions among the pro-inflammatory cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in the early stage of ischemic reperfusion are not yet completely understood. In this study, we examined the early mRNA expressions of pro-inflammatory cytokines in the ischemic hippocampus after 30 min of bilateral common carotid artery occlusion in C57BL/6J wild-type (WT) and TNF-α, IL-1α/β or IL-6 gene knockout (KO) mice utilizing real-time polymerase chain reaction. The mRNA expressions of the pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were significantly induced in ischemic WT mice compared with in the sham-operated mice. These increases peaked at 3 to 24 h for TNF-α, at 12 h for IL-1β, and at 6 to 24 h for IL-6 after ischemia. The pattern of temporal expression of the cytokine mRNAs in ischemic gene KO mice, however, differed from that in WT mice. The TNF-α mRNA expression showed a similar temporal expression pattern in IL-6 KO mice compared to in WT mice following ischemic reperfusion, and the levels at all time points were lower than in WT mice. The IL-1β mRNA level was very low in ischemic TNF-α KO mice and IL-6 KO mice in spite of a small peak observed in both at 24 h. The IL-6 mRNA level was significantly upregulated at all time points in both ischemic WT and TNF-α KO mice; however, the peak was delayed by 12-h in IL-1α/β KO mice. In conclusion, the present study indicates that the rapid increases in cytokine levels are interdependent, interactive, and possibly modulate each other in the mouse hippocampus after transient global ischemia.

UR - http://www.scopus.com/inward/record.url?scp=29244478608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29244478608&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.08.072

DO - 10.1016/j.neulet.2005.08.072

M3 - Article

C2 - 16356636

AN - SCOPUS:29244478608

VL - 393

SP - 122

EP - 126

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2-3

ER -