EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells

Wenjing Li, Tomohiro Miki, Takashi Watanabe, Mai Kakeno, Ikuko Sugiyama, Kozo Kaibuchi, Gohta Goshima

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Highly conserved EB1 family proteins bind to the growing ends of microtubules, recruit multiple cargo proteins, and are critical for making dynamic microtubules in vivo. However, it is unclear how these master regulators of microtubule plus ends promote microtubule dynamics. In this paper, we identify a novel EB1 cargo protein, Sentin. Sentin depletion in Drosophila melanogaster S2 cells, similar to EB1 depletion, resulted in an increase in microtubule pausing and led to the formation of shorter spindles, without displacing EB1 from growing microtubules. We demonstrate that Sentin's association with EB1 was critical for its plus end localization and function. Furthermore, the EB1 phenotype was rescued by expressing an EBN-Sentin fusion protein in which the C-terminal cargo-binding region of EB1 is replaced with Sentin. Knockdown of Sentin attenuated plus end accumulation of Msps (mini spindles), the orthologue of XMAP215 microtubule polymerase. These results indicate that EB1 promotes dynamic microtubule behavior by recruiting the cargo protein Sentin and possibly also a microtubule polymerase to the microtubule tip.

Original languageEnglish
Pages (from-to)973-983
Number of pages11
JournalJournal of Cell Biology
Volume193
Issue number6
DOIs
Publication statusPublished - 13-06-2011

All Science Journal Classification (ASJC) codes

  • Cell Biology

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