Effect of amurinone on human pulmonary artery in vitro

S. Ono, S. Ueda, T. Sakuma, M. Noda, T. Tabata, Y. Hoshikawa, Y. Ashino, T. Tanita, K. Koike, S. Fujimura

Research output: Contribution to journalArticle

Abstract

We studied the effect of amurinone, a phosphodiesterase inhibitor, on human pulmonary arterial smooth muscle in vitro. Amurinone caused dose-dependent relaxation of pulmonary arterial strips precontracted with 60 mM KCl. Preincubation with either meclofenamate (31 μM), a cyclooxygenase inhibitor, or L-N(G)-nitroarginine (100 μM), an endothelium-derived relaxing factor (EDRF) production inhibitor, failed to inhibit amurinone-induced pulmonary vasodilation. The cyclic AMP (cAMP) levels in the supernatant of the lung vessel homogenates significantly increased after incubation with amurinone. These results indicate that amurinone causes relaxation of human pulmonary artery in vitro, and suggest a role for cAMP in the mechanisms of amurinone-induced pulmonary vasodilation.

Original languageEnglish
Pages (from-to)650-654
Number of pages5
JournalJapanese Journal of Thoracic Diseases
Volume32
Issue number7
Publication statusPublished - 01-01-1994

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Pulmonary Artery
Lung
Vasodilation
Cyclic AMP
Meclofenamic Acid
Endothelium-Dependent Relaxing Factors
Phosphodiesterase Inhibitors
Cyclooxygenase Inhibitors
Nitroarginine
Smooth Muscle
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Ono, S., Ueda, S., Sakuma, T., Noda, M., Tabata, T., Hoshikawa, Y., ... Fujimura, S. (1994). Effect of amurinone on human pulmonary artery in vitro. Japanese Journal of Thoracic Diseases, 32(7), 650-654.
Ono, S. ; Ueda, S. ; Sakuma, T. ; Noda, M. ; Tabata, T. ; Hoshikawa, Y. ; Ashino, Y. ; Tanita, T. ; Koike, K. ; Fujimura, S. / Effect of amurinone on human pulmonary artery in vitro. In: Japanese Journal of Thoracic Diseases. 1994 ; Vol. 32, No. 7. pp. 650-654.
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Ono, S, Ueda, S, Sakuma, T, Noda, M, Tabata, T, Hoshikawa, Y, Ashino, Y, Tanita, T, Koike, K & Fujimura, S 1994, 'Effect of amurinone on human pulmonary artery in vitro', Japanese Journal of Thoracic Diseases, vol. 32, no. 7, pp. 650-654.

Effect of amurinone on human pulmonary artery in vitro. / Ono, S.; Ueda, S.; Sakuma, T.; Noda, M.; Tabata, T.; Hoshikawa, Y.; Ashino, Y.; Tanita, T.; Koike, K.; Fujimura, S.

In: Japanese Journal of Thoracic Diseases, Vol. 32, No. 7, 01.01.1994, p. 650-654.

Research output: Contribution to journalArticle

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Ono S, Ueda S, Sakuma T, Noda M, Tabata T, Hoshikawa Y et al. Effect of amurinone on human pulmonary artery in vitro. Japanese Journal of Thoracic Diseases. 1994 Jan 1;32(7):650-654.