TY - JOUR
T1 - Effect of anticoagulants in colorimetric assay for basic carboxypeptidases
AU - Komura, Hidefumi
AU - Obata, Kyoko
AU - Campbell, William
AU - Yumoto, Miho
AU - Shimomura, Yasuyo
AU - Katsuya, Hirotada
AU - Okada, Noriko
AU - Okada, Hidechika
PY - 2002
Y1 - 2002
N2 - Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is convenient for determining hippuric acid generated by CP, we noticed that some anticoagulants, such as citrate, interfere with the color development of the reagents used. EDTA and heparin provide an appropriate value. EGTA used as anticoagulant also provides an appropriate value. Therefore, concentration of citrate in samples should be controlled to be constant for background subtraction.
AB - Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is convenient for determining hippuric acid generated by CP, we noticed that some anticoagulants, such as citrate, interfere with the color development of the reagents used. EDTA and heparin provide an appropriate value. EGTA used as anticoagulant also provides an appropriate value. Therefore, concentration of citrate in samples should be controlled to be constant for background subtraction.
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U2 - 10.1111/j.1348-0421.2002.tb02667.x
DO - 10.1111/j.1348-0421.2002.tb02667.x
M3 - Article
C2 - 11939576
AN - SCOPUS:0036186744
SN - 0385-5600
VL - 46
SP - 115
EP - 117
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 2
ER -