Effect of anticoagulants in colorimetric assay for basic carboxypeptidases

Hidefumi Komura, Kyoko Obata, William Campbell, Miho Yumoto, Yasuyo Shimomura, Hirotada Katsuya, Noriko Okada, Hidechika Okada

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is convenient for determining hippuric acid generated by CP, we noticed that some anticoagulants, such as citrate, interfere with the color development of the reagents used. EDTA and heparin provide an appropriate value. EGTA used as anticoagulant also provides an appropriate value. Therefore, concentration of citrate in samples should be controlled to be constant for background subtraction.

Original languageEnglish
Pages (from-to)115-117
Number of pages3
JournalMICROBIOLOGY and IMMUNOLOGY
Volume46
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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