Effect of chlorphentermine on the pulmonary disposition of norepinephrine in the isolated perfused rabbit lung

Linda S. Angevine, Toshitaka Nabeshima, Ing K. Ho, Harihara M. Mehendale

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cblorphentermine (CP) has been noted to cause primary pulmonary hypertension both clinically and experimentally. It was postulated that CP might affect the pulmonary clearance of endogenous vasoactive substances such as norepinephrine (NE). The uptake and metabolism of 14C-NE were followed in artificially ventilated isolated perfused rabbit lung preparations using a constituted perfusate with initial NE concentrations of 5 μg/100 ml. Perfusate samples were analyzed for total radioactivity, metabolites, and parent compound. Preloading the lungs with 0.25 mM CP significantly increased the concentration of total radioactivity, deaminated products, and decreased the concentration of normetanephrine in the perfusate. In addition, the accumulation of total radioactivity in the lung tissue after 60 min of perfusion was significantly decreased in CP-treated lungs. The proportion of deaminated metabolites in the lung tissue was slightly decreased while the percent of normetanephrine, and parent compound were significantly increased by the CP treatment. CP (0.1 mM) also inhibited the in vitro metabolism of NE by 79%. These results provide experimental evidence in support of a hindered pulmonary clearance of circulating NE by CP.

Original languageEnglish
Pages (from-to)71-84
Number of pages14
JournalExperimental Lung Research
Volume2
Issue number2
DOIs
Publication statusPublished - 01-01-1981
Externally publishedYes

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Chlorphentermine
Norepinephrine
Rabbits
Radioactivity
Lung
Normetanephrine
Metabolites
Metabolism
Tissue
Perfusion

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

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abstract = "Cblorphentermine (CP) has been noted to cause primary pulmonary hypertension both clinically and experimentally. It was postulated that CP might affect the pulmonary clearance of endogenous vasoactive substances such as norepinephrine (NE). The uptake and metabolism of 14C-NE were followed in artificially ventilated isolated perfused rabbit lung preparations using a constituted perfusate with initial NE concentrations of 5 μg/100 ml. Perfusate samples were analyzed for total radioactivity, metabolites, and parent compound. Preloading the lungs with 0.25 mM CP significantly increased the concentration of total radioactivity, deaminated products, and decreased the concentration of normetanephrine in the perfusate. In addition, the accumulation of total radioactivity in the lung tissue after 60 min of perfusion was significantly decreased in CP-treated lungs. The proportion of deaminated metabolites in the lung tissue was slightly decreased while the percent of normetanephrine, and parent compound were significantly increased by the CP treatment. CP (0.1 mM) also inhibited the in vitro metabolism of NE by 79{\%}. These results provide experimental evidence in support of a hindered pulmonary clearance of circulating NE by CP.",
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Effect of chlorphentermine on the pulmonary disposition of norepinephrine in the isolated perfused rabbit lung. / Angevine, Linda S.; Nabeshima, Toshitaka; Ho, Ing K.; Mehendale, Harihara M.

In: Experimental Lung Research, Vol. 2, No. 2, 01.01.1981, p. 71-84.

Research output: Contribution to journalArticle

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