Effect of dietary vitamin E supplementation on liver oxidative damage in rats with water-immersion restraint stress

Yoshiji Ohta, Koji Yashiro, Koji Ohashi, Yosuke Horikoshi, Chiaki Kusumoto, Tatsuya Matsura, Kenji Fukuzawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU a-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver a-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver a-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ9 and reduced CoQ9 concentrations in VESD-fed rats but decreased liver total CoQ9, reduced CoQ9, and reduced CoQ10 concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalJournal of Nutritional Science and Vitaminology
Volume61
Issue number2
DOIs
Publication statusPublished - 08-06-2015

Fingerprint

Immersion
Vitamin E
Diet
Water
Liver
coenzyme Q10
Tocopherols
Lipid Peroxides
Ascorbic Acid
Corticosterone
Dietary Supplements
Transaminases
Serum
L-Lactate Dehydrogenase
Adrenocorticotropic Hormone
Glutathione
ubiquinone 9

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Ohta, Yoshiji ; Yashiro, Koji ; Ohashi, Koji ; Horikoshi, Yosuke ; Kusumoto, Chiaki ; Matsura, Tatsuya ; Fukuzawa, Kenji. / Effect of dietary vitamin E supplementation on liver oxidative damage in rats with water-immersion restraint stress. In: Journal of Nutritional Science and Vitaminology. 2015 ; Vol. 61, No. 2. pp. 113-122.
@article{f29c72b2c5a44a9c9a9f58561763f856,
title = "Effect of dietary vitamin E supplementation on liver oxidative damage in rats with water-immersion restraint stress",
abstract = "We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU a-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver a-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver a-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ9 and reduced CoQ9 concentrations in VESD-fed rats but decreased liver total CoQ9, reduced CoQ9, and reduced CoQ10 concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.",
author = "Yoshiji Ohta and Koji Yashiro and Koji Ohashi and Yosuke Horikoshi and Chiaki Kusumoto and Tatsuya Matsura and Kenji Fukuzawa",
year = "2015",
month = "6",
day = "8",
doi = "10.3177/jnsv.61.113",
language = "English",
volume = "61",
pages = "113--122",
journal = "Journal of Nutritional Science and Vitaminology",
issn = "0301-4800",
publisher = "Center for Academic Publications Japan",
number = "2",

}

Effect of dietary vitamin E supplementation on liver oxidative damage in rats with water-immersion restraint stress. / Ohta, Yoshiji; Yashiro, Koji; Ohashi, Koji; Horikoshi, Yosuke; Kusumoto, Chiaki; Matsura, Tatsuya; Fukuzawa, Kenji.

In: Journal of Nutritional Science and Vitaminology, Vol. 61, No. 2, 08.06.2015, p. 113-122.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of dietary vitamin E supplementation on liver oxidative damage in rats with water-immersion restraint stress

AU - Ohta, Yoshiji

AU - Yashiro, Koji

AU - Ohashi, Koji

AU - Horikoshi, Yosuke

AU - Kusumoto, Chiaki

AU - Matsura, Tatsuya

AU - Fukuzawa, Kenji

PY - 2015/6/8

Y1 - 2015/6/8

N2 - We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU a-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver a-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver a-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ9 and reduced CoQ9 concentrations in VESD-fed rats but decreased liver total CoQ9, reduced CoQ9, and reduced CoQ10 concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.

AB - We examined how dietary supplementation of vitamin E protects against liver oxidative damage in rats with water-immersion restraint stress (WIRS). Before WIRS exposure, rats received a normal diet (ND) or vitamin E-supplemented diet (VESD) (500 IU a-tocopherol/kg diet) at a mean dose of 15 g/animal/d for 4 wk. The two diet groups had serum transaminases and lactate dehydrogenase activities and adrenocorticotropic hormone, corticosterone, and glucose levels to a similar extent. VESD-fed rats had higher liver a-tocopherol concentrations and lower liver ascorbic acid, total coenzyme Q9 (CoQ9), reduced CoQ9, reduced CoQ10, and lipid peroxide (LPO) concentrations than ND-fed rats. When the two diet groups were exposed to 6 h of WIRS, the serum liver cell damage index enzyme activities increased more greatly in ND-fed rats than in VESD-fed rats but the serum stress marker levels increased to a similar extent. The WIRS exposure caused no change in liver LPO concentration with the further increase in liver a-tocopherol concentration in VESD-fed rats but increased liver LPO concentration without changing liver a-tocopherol concentration in ND-fed rats. Upon the WIRS exposure, liver reduced glutathione concentration decreased with the further decrease in liver ascorbic acid concentration in VESD-fed rats and those concentrations decreased in ND-fed rats. The WIRS exposure recovered the decreased liver total CoQ9 and reduced CoQ9 concentrations in VESD-fed rats but decreased liver total CoQ9, reduced CoQ9, and reduced CoQ10 concentrations in ND-fed rats. These results indicate that dietary vitamin E supplementation protects against liver oxidative damage without affecting the stress response in rats with WIRS.

UR - http://www.scopus.com/inward/record.url?scp=84930742351&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930742351&partnerID=8YFLogxK

U2 - 10.3177/jnsv.61.113

DO - 10.3177/jnsv.61.113

M3 - Article

VL - 61

SP - 113

EP - 122

JO - Journal of Nutritional Science and Vitaminology

JF - Journal of Nutritional Science and Vitaminology

SN - 0301-4800

IS - 2

ER -