TY - JOUR
T1 - Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells
AU - Atsushi, Suzuki
AU - Yutaka, Oisoa
AU - Osamu, Kozawab
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/11
Y1 - 1994/11
N2 - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
AB - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
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U2 - 10.1016/0303-7207(94)90169-4
DO - 10.1016/0303-7207(94)90169-4
M3 - Article
C2 - 7859925
AN - SCOPUS:0028077539
VL - 105
SP - 193
EP - 196
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 2
ER -