Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

Atsushi Suzuki, Oisoa Yutaka, Kozawab Osamu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalMolecular and Cellular Endocrinology
Volume105
Issue number2
DOIs
Publication statusPublished - 01-01-1994
Externally publishedYes

Fingerprint

Phospholipase D
Osteoblasts
Endothelin-1
Choline
Protein Kinase C
Inositol Phosphates
Staurosporine
Pertussis Toxin
Phorbol Esters
Tetradecanoylphorbol Acetate
Phosphatidylcholines
Acetates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

@article{715de2538ac14bcd8d0325cb4fa07b7e,
title = "Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells",
abstract = "We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.",
author = "Atsushi Suzuki and Oisoa Yutaka and Kozawab Osamu",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0303-7207(94)90169-4",
language = "English",
volume = "105",
pages = "193--196",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells. / Suzuki, Atsushi; Yutaka, Oisoa; Osamu, Kozawab.

In: Molecular and Cellular Endocrinology, Vol. 105, No. 2, 01.01.1994, p. 193-196.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

AU - Suzuki, Atsushi

AU - Yutaka, Oisoa

AU - Osamu, Kozawab

PY - 1994/1/1

Y1 - 1994/1/1

N2 - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

AB - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

UR - http://www.scopus.com/inward/record.url?scp=0028077539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028077539&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(94)90169-4

DO - 10.1016/0303-7207(94)90169-4

M3 - Article

VL - 105

SP - 193

EP - 196

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 2

ER -