Abstract
We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
Original language | English |
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Pages (from-to) | 193-196 |
Number of pages | 4 |
Journal | Molecular and Cellular Endocrinology |
Volume | 105 |
Issue number | 2 |
DOIs | |
Publication status | Published - 11-1994 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Endocrinology