Abstract
We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
| Original language | English |
|---|---|
| Pages (from-to) | 193-196 |
| Number of pages | 4 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 105 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 01-01-1994 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Endocrinology
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Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells. / Suzuki, Atsushi; Yutaka, Oisoa; Osamu, Kozawab.
In: Molecular and Cellular Endocrinology, Vol. 105, No. 2, 01.01.1994, p. 193-196.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells
AU - Suzuki, Atsushi
AU - Yutaka, Oisoa
AU - Osamu, Kozawab
PY - 1994/1/1
Y1 - 1994/1/1
N2 - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
AB - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC 50 10 nM) as well as that of inositol phosphates (EC 50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.
UR - http://www.scopus.com/inward/record.url?scp=0028077539&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028077539&partnerID=8YFLogxK
U2 - 10.1016/0303-7207(94)90169-4
DO - 10.1016/0303-7207(94)90169-4
M3 - Article
C2 - 7859925
AN - SCOPUS:0028077539
VL - 105
SP - 193
EP - 196
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 2
ER -