Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

Suzuki Atsushi; Oisoa Yutaka; Kozawab Osamu

doi:10.1016/0303-7207(94)90169-4

Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

Suzuki Atsushi
, Oisoa Yutaka
, Kozawab Osamu

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC₅₀ 10 nM) as well as that of inositol phosphates (EC₅₀ 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

Original language	English
Pages (from-to)	193-196
Number of pages	4
Journal	Molecular and Cellular Endocrinology
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(94)90169-4
Publication status	Published - 11-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Endocrinology

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title = "Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells",

abstract = "We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.",

author = "Suzuki Atsushi and Oisoa Yutaka and Kozawab Osamu",

year = "1994",

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doi = "10.1016/0303-7207(94)90169-4",

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T1 - Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

AU - Atsushi, Suzuki

AU - Yutaka, Oisoa

AU - Osamu, Kozawab

PY - 1994/11

Y1 - 1994/11

N2 - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

AB - We examined the effect of endothelin-1 (ET-1) on phosphatidylcholine-hydrolyzing phospholipase D activity in osteoblast-like MC3T3-E1 cells. ET-1 stimulated the formation of choline (EC50 10 nM) as well as that of inositol phosphates (EC50 1.2 nM). The effects of ET-1 and 12-0-tetradecanoylphorbol-13-acetate, a protein kinase C-activating phorbol ester, were additive. Staurosporine enhanced the ET-1-induced formation of choline. NaF or pertussis toxin were ineffective. The results indicate that ET-1 activates phospholipase D independent of protein kinase C in osteoblast-like cells.

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U2 - 10.1016/0303-7207(94)90169-4

DO - 10.1016/0303-7207(94)90169-4

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SN - 0303-7207

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Effect of endothelin-1 on phospholipase D activity in osteoblast- like cells

Abstract

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