TY - JOUR
T1 - Effect of erythromycin on matrix metalloproteinase-9 and cell migration
AU - Hashimoto, Naozumi
AU - Kawabe, Tsutomu
AU - Hara, Toru
AU - Imaizumi, Kazuyoshi
AU - Wakayama, Hisashi
AU - Saito, Hidehiko
AU - Shimokata, Kaoru
AU - Hasegawa, Yoshinori
PY - 2001
Y1 - 2001
N2 - Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.
AB - Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.
UR - http://www.scopus.com/inward/record.url?scp=0035098059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035098059&partnerID=8YFLogxK
U2 - 10.1067/mlc.2001.112726
DO - 10.1067/mlc.2001.112726
M3 - Article
C2 - 11241027
AN - SCOPUS:0035098059
VL - 137
SP - 176
EP - 183
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 3
ER -