Effect of erythromycin on matrix metalloproteinase-9 and cell migration

Naozumi Hashimoto, Tsutomu Kawabe, Toru Hara, Kazuyoshi Imaizumi, Hisashi Wakayama, Hidehiko Saito, Kaoru Shimokata, Yoshinori Hasegawa

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Erythromycin (EM) has an anti-inflammatory effect that may account for its clinical benefit in the treatment of chronic inflammatory diseases such as diffuse panbronchiolitis. To investigate the mechanism of this anti-inflammatory effect, we studied the relationship between the concentration of EM and matrix metalloproteinase-9 (MMP-9) activity, which is important in cell migration. We showed that EM suppressed the gelatinolytic activity of U937 cell-derived MMP-9 by using gelatin zymography, showed that expressions of MMP-9 protein and MMP-9 mRNA were down-regulated by EM in a dose-dependent manner, and showed that U937 migration was also suppressed by EM. We also demonstrated that EM treatment suppressed the gelatinolytic activity of MMP-9 in spleen macrophages. These findings suggest that the suppressive effect of EM on MMP-9 activity is one of the anti-inflammatory mechanisms that inhibit the migration of inflammatory cells into the inflammatory site.

Original languageEnglish
Pages (from-to)176-183
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume137
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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