Effect of Glutamine on Protein Synthesis in Isolated Intestinal Epithelial Cells

Takashi Higashiguchi, Per Olof Hasselgren, Kenneth Wagner, Josef E. Fischer

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The influence of glutamine on protein synthesis in small-bowel enterocytes was tested. Enterocytes were isolated from different levels of the villi of rat jejunum and were incubated in the presence of different glutamine concentrations, up to 3.4 mmol/L. Protein synthesis was determined by measuring incorporation of 3H-phenylalanine into trichloroacetic acid-precipitated proteins. Glutamine, but no other amino acids, stimulated protein synthesis in enterocytes from all levels of the villi. A maximal effect was noted at a glutamine concentration of 0.67 mmol/L, which is the normal plasma concentration. The amino acid stimulated the synthesis of both secreted and nonsecreted proteins. The stimulatory effect of glutamine on protein synthesis was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine and was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate. The results suggest that glutamine stimulates protein synthesis in small-bowel enterocytes and that this effect of glutamine is related to provision of energy. The findings are important because they suggest that increased protein synthesis may be one of the mechanisms by which glutamine exerts its protective effect on gut mucosa during critical illness.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalJournal of Parenteral and Enteral Nutrition
Volume17
Issue number4
DOIs
Publication statusPublished - 01-01-1993
Externally publishedYes

Fingerprint

Glutamine
Epithelial Cells
Enterocytes
Proteins
Diazooxonorleucine
Glutaminase
Amino Acids
Trichloroacetic Acid
3-Hydroxybutyric Acid
Jejunum
Phenylalanine
Critical Illness
Mucous Membrane

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Higashiguchi, Takashi ; Hasselgren, Per Olof ; Wagner, Kenneth ; Fischer, Josef E. / Effect of Glutamine on Protein Synthesis in Isolated Intestinal Epithelial Cells. In: Journal of Parenteral and Enteral Nutrition. 1993 ; Vol. 17, No. 4. pp. 307-314.
@article{8a4dbbcfd4bc4423a246af08152ad4c8,
title = "Effect of Glutamine on Protein Synthesis in Isolated Intestinal Epithelial Cells",
abstract = "The influence of glutamine on protein synthesis in small-bowel enterocytes was tested. Enterocytes were isolated from different levels of the villi of rat jejunum and were incubated in the presence of different glutamine concentrations, up to 3.4 mmol/L. Protein synthesis was determined by measuring incorporation of 3H-phenylalanine into trichloroacetic acid-precipitated proteins. Glutamine, but no other amino acids, stimulated protein synthesis in enterocytes from all levels of the villi. A maximal effect was noted at a glutamine concentration of 0.67 mmol/L, which is the normal plasma concentration. The amino acid stimulated the synthesis of both secreted and nonsecreted proteins. The stimulatory effect of glutamine on protein synthesis was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine and was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate. The results suggest that glutamine stimulates protein synthesis in small-bowel enterocytes and that this effect of glutamine is related to provision of energy. The findings are important because they suggest that increased protein synthesis may be one of the mechanisms by which glutamine exerts its protective effect on gut mucosa during critical illness.",
author = "Takashi Higashiguchi and Hasselgren, {Per Olof} and Kenneth Wagner and Fischer, {Josef E.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1177/0148607193017004307",
language = "English",
volume = "17",
pages = "307--314",
journal = "Journal of Parenteral and Enteral Nutrition",
issn = "0148-6071",
publisher = "SAGE Publications Inc.",
number = "4",

}

Effect of Glutamine on Protein Synthesis in Isolated Intestinal Epithelial Cells. / Higashiguchi, Takashi; Hasselgren, Per Olof; Wagner, Kenneth; Fischer, Josef E.

In: Journal of Parenteral and Enteral Nutrition, Vol. 17, No. 4, 01.01.1993, p. 307-314.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Glutamine on Protein Synthesis in Isolated Intestinal Epithelial Cells

AU - Higashiguchi, Takashi

AU - Hasselgren, Per Olof

AU - Wagner, Kenneth

AU - Fischer, Josef E.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - The influence of glutamine on protein synthesis in small-bowel enterocytes was tested. Enterocytes were isolated from different levels of the villi of rat jejunum and were incubated in the presence of different glutamine concentrations, up to 3.4 mmol/L. Protein synthesis was determined by measuring incorporation of 3H-phenylalanine into trichloroacetic acid-precipitated proteins. Glutamine, but no other amino acids, stimulated protein synthesis in enterocytes from all levels of the villi. A maximal effect was noted at a glutamine concentration of 0.67 mmol/L, which is the normal plasma concentration. The amino acid stimulated the synthesis of both secreted and nonsecreted proteins. The stimulatory effect of glutamine on protein synthesis was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine and was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate. The results suggest that glutamine stimulates protein synthesis in small-bowel enterocytes and that this effect of glutamine is related to provision of energy. The findings are important because they suggest that increased protein synthesis may be one of the mechanisms by which glutamine exerts its protective effect on gut mucosa during critical illness.

AB - The influence of glutamine on protein synthesis in small-bowel enterocytes was tested. Enterocytes were isolated from different levels of the villi of rat jejunum and were incubated in the presence of different glutamine concentrations, up to 3.4 mmol/L. Protein synthesis was determined by measuring incorporation of 3H-phenylalanine into trichloroacetic acid-precipitated proteins. Glutamine, but no other amino acids, stimulated protein synthesis in enterocytes from all levels of the villi. A maximal effect was noted at a glutamine concentration of 0.67 mmol/L, which is the normal plasma concentration. The amino acid stimulated the synthesis of both secreted and nonsecreted proteins. The stimulatory effect of glutamine on protein synthesis was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine and was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate. The results suggest that glutamine stimulates protein synthesis in small-bowel enterocytes and that this effect of glutamine is related to provision of energy. The findings are important because they suggest that increased protein synthesis may be one of the mechanisms by which glutamine exerts its protective effect on gut mucosa during critical illness.

UR - http://www.scopus.com/inward/record.url?scp=0027323361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027323361&partnerID=8YFLogxK

U2 - 10.1177/0148607193017004307

DO - 10.1177/0148607193017004307

M3 - Article

VL - 17

SP - 307

EP - 314

JO - Journal of Parenteral and Enteral Nutrition

JF - Journal of Parenteral and Enteral Nutrition

SN - 0148-6071

IS - 4

ER -