Effect of high molecular weight hyaluronan on cartilage degeneration in a rabbit model of osteoarthritis

T. Kikuchi, Harumoto Yamada, M. Shimmei

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

The effects of high molecular weight hyaluronan (HA) on cartilage degeneration were investigated in a partial menisectomy model of osteoarthritis (OA) in the rabbit knee. This study compared HA80 (0.8x106 Da, 1%), HA190 (1.9x106 Da, 0.01-1%) and saline. HA (0.1 ml/kg) or saline were injected intra-articularly twice a week immediately after surgery. Degenerative changes in femoral and tibial cartilages were graded histopathologically 2 and 4 weeks after surgery. Two weeks after surgery, HA190, only when used at a 1% concentration, resulted in a dramatic inhibition of cartilage degeneration in both the femoral condyle and the tibial plateau (P < 0.01). Two weeks after surgery, the protection against cartilage degeneration was significantly (P < 0.05) greater with HA190 than with HA80. Four weeks after surgery, only the femoral cartilage degeneration was significantly and similarly inhibited with HA190 (P < 0.01) and HA80 (P < 0.05). Scanning electron micrographs of femoral cartilage showed that cartilage degeneration was less severe with HA190 than with saline. These results might suggest that, in the rabbit model, intra-articular administration of higher molecular weight HA is more effective than lower molecular weight HA in inhibiting cartilage degeneration in early OA.

Original languageEnglish
Pages (from-to)99-110
Number of pages12
JournalOsteoarthritis and Cartilage
Volume4
Issue number2
DOIs
Publication statusPublished - 01-01-1996
Externally publishedYes

Fingerprint

Cartilage
Hyaluronic Acid
Osteoarthritis
Molecular Weight
Molecular weight
Rabbits
Surgery
Thigh
Knee
Joints
Electrons
Scanning
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Biomedical Engineering
  • Orthopedics and Sports Medicine

Cite this

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abstract = "The effects of high molecular weight hyaluronan (HA) on cartilage degeneration were investigated in a partial menisectomy model of osteoarthritis (OA) in the rabbit knee. This study compared HA80 (0.8x106 Da, 1{\%}), HA190 (1.9x106 Da, 0.01-1{\%}) and saline. HA (0.1 ml/kg) or saline were injected intra-articularly twice a week immediately after surgery. Degenerative changes in femoral and tibial cartilages were graded histopathologically 2 and 4 weeks after surgery. Two weeks after surgery, HA190, only when used at a 1{\%} concentration, resulted in a dramatic inhibition of cartilage degeneration in both the femoral condyle and the tibial plateau (P < 0.01). Two weeks after surgery, the protection against cartilage degeneration was significantly (P < 0.05) greater with HA190 than with HA80. Four weeks after surgery, only the femoral cartilage degeneration was significantly and similarly inhibited with HA190 (P < 0.01) and HA80 (P < 0.05). Scanning electron micrographs of femoral cartilage showed that cartilage degeneration was less severe with HA190 than with saline. These results might suggest that, in the rabbit model, intra-articular administration of higher molecular weight HA is more effective than lower molecular weight HA in inhibiting cartilage degeneration in early OA.",
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Effect of high molecular weight hyaluronan on cartilage degeneration in a rabbit model of osteoarthritis. / Kikuchi, T.; Yamada, Harumoto; Shimmei, M.

In: Osteoarthritis and Cartilage, Vol. 4, No. 2, 01.01.1996, p. 99-110.

Research output: Contribution to journalArticle

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