Abstract
Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.
| Original language | English |
|---|---|
| Pages (from-to) | 380-385.e1 |
| Journal | Annals of Allergy, Asthma and Immunology |
| Volume | 110 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-01-2013 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Pulmonary and Respiratory Medicine
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Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood . / Nomura, Takayasu; Tsuge, Ikuya; Inuo, Chisato; Nakajima, Yoichi; Tanaka, Kenichi; Naruse, Norihiko; Suzuki, Satoko; Ando, Hitoshi; Kondo, Yasuto; Saitoh, Shinji; Urisu, Atsuo.
In: Annals of Allergy, Asthma and Immunology, Vol. 110, No. 5, 01.01.2013, p. 380-385.e1.Research output: Contribution to journal › Article
TY - JOUR
T1 - Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood
AU - Nomura, Takayasu
AU - Tsuge, Ikuya
AU - Inuo, Chisato
AU - Nakajima, Yoichi
AU - Tanaka, Kenichi
AU - Naruse, Norihiko
AU - Suzuki, Satoko
AU - Ando, Hitoshi
AU - Kondo, Yasuto
AU - Saitoh, Shinji
AU - Urisu, Atsuo
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.
AB - Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.
UR - http://www.scopus.com/inward/record.url?scp=84876682487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876682487&partnerID=8YFLogxK
U2 - 10.1016/j.anai.2013.02.015
DO - 10.1016/j.anai.2013.02.015
M3 - Article
C2 - 23622011
AN - SCOPUS:84876682487
VL - 110
SP - 380-385.e1
JO - Annals of Allergy, Asthma and Immunology
JF - Annals of Allergy, Asthma and Immunology
SN - 1081-1206
IS - 5
ER -