Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood

Takayasu Nomura, Ikuya Tsuge, Chisato Inuo, Yoichi Nakajima, Kenichi Tanaka, Norihiko Naruse, Satoko Suzuki, Hitoshi Ando, Yasuto Kondo, Shinji Saitoh, Atsuo Urisu

Research output: Contribution to journalArticle

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Abstract

Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.

Original languageEnglish
Pages (from-to)380-385.e1
JournalAnnals of Allergy, Asthma and Immunology
Volume110
Issue number5
DOIs
Publication statusPublished - 01-01-2013

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Cryptomeria
Immunologic Desensitization
Immunotherapy
Viral Tumor Antigens
Interleukin-5
Pollen
Interleukin-4
Antigens
Control Groups
Seasonal Allergic Rhinitis
Interleukin-17
Interleukin-10
Interferons
Young Adult
Flow Cytometry
Tumor Necrosis Factor-alpha
Cell Proliferation
Cytokines

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

Nomura, Takayasu ; Tsuge, Ikuya ; Inuo, Chisato ; Nakajima, Yoichi ; Tanaka, Kenichi ; Naruse, Norihiko ; Suzuki, Satoko ; Ando, Hitoshi ; Kondo, Yasuto ; Saitoh, Shinji ; Urisu, Atsuo. / Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood In: Annals of Allergy, Asthma and Immunology. 2013 ; Vol. 110, No. 5. pp. 380-385.e1.
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abstract = "Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.",
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Nomura, T, Tsuge, I, Inuo, C, Nakajima, Y, Tanaka, K, Naruse, N, Suzuki, S, Ando, H, Kondo, Y, Saitoh, S & Urisu, A 2013, ' Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood ', Annals of Allergy, Asthma and Immunology, vol. 110, no. 5, pp. 380-385.e1. https://doi.org/10.1016/j.anai.2013.02.015

Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood . / Nomura, Takayasu; Tsuge, Ikuya; Inuo, Chisato; Nakajima, Yoichi; Tanaka, Kenichi; Naruse, Norihiko; Suzuki, Satoko; Ando, Hitoshi; Kondo, Yasuto; Saitoh, Shinji; Urisu, Atsuo.

In: Annals of Allergy, Asthma and Immunology, Vol. 110, No. 5, 01.01.2013, p. 380-385.e1.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Japanese cedar specific immunotherapy on allergen-specific T H 2 cells in peripheral blood

AU - Nomura, Takayasu

AU - Tsuge, Ikuya

AU - Inuo, Chisato

AU - Nakajima, Yoichi

AU - Tanaka, Kenichi

AU - Naruse, Norihiko

AU - Suzuki, Satoko

AU - Ando, Hitoshi

AU - Kondo, Yasuto

AU - Saitoh, Shinji

AU - Urisu, Atsuo

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.

AB - Background: The involvement of a shift from T H 2 to T H 1 responses in peripheral blood in pollen subcutaneous immunotherapy (SCIT) has been contentious, partly because of difficulties analyzing antigen-specific T H cells. Objectives: To use recent technical advances to establish a more direct and simple method to analyze antigen-specific T H cells and to clarify the involvement of a T H 2/T H 1 shift in peripheral blood in pollen specific immunotherapy. Methods: After short-term (6-hour) antigen stimulation, antigen-specific T H cells in peripheral blood of Japanese children and young adults with Japanese cedar pollinosis undergoing SCIT were analyzed by multicolor flow cytometry for the presence of the activation marker CD154 and intracellular cytokines. Results: Twenty-eight patients between 5 and 22 years of age were enrolled in the study; 22 had started SCIT after enrolling in the study (SCIT group), and the remaining 6 were planning to start SCIT in the next off-season (control group). The number of Japanese cedar-specific interleukin (IL) 5-, IL-4-, interferon γ-, IL-17A-, IL-10-, and tumor necrosis factor α-producing T H cells without antigen-driven cell proliferation was determined. The seasonal increase in the number of Japanese cedar-specific IL-5- and IL-4-producing T H cells seen in the control group was suppressed in the SCIT group (P <.005 and <.001, respectively). Conclusion: We report a powerful method for the analysis of antigen-specific T H cells in peripheral blood. This method will contribute to our understanding of immune mechanisms of immunotherapy and help us develop more sophisticated allergen specific immunotherapy.

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