Effect of lipopolysaccharide on the gene expression of the enzymes involved in tetrahydrobiopterin de novo biosynthesis in murine neuroblastoma cell line N1E-115

Keiji Mori, Akira Nakashima, Toshiharu Nagatsu, Akira Ota

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The amounts of messenger RNA for three enzymes, namely guanosine triphosphate (GTP) cyclohydrolase I, 6-pyruvoyltetrahydropterin synthase, and sepiapterin reductase, all of which are involved in the de novo biosynthesis of (6R)-L-erythrodihydroxypropyl-2-amino-4-hydroxy-5,6,7,8- tetrahydropteridine (BH4) from GTP, were measured quantitatively in murine neuroblastoma cell line N1E-115 by the competitive polymerase chain reaction (PCR) technique after reverse transcription using a heterologous DNA fragment as an internal standard. Twenty-four hour activation of this cell line with 1 μg/ml lipopolysaccharide resulted in statistically significant increases in the amounts of the messages of all three enzymes. Our data suggest that lipopolysaccharide can activate the intrinsic pathway resulting in the enhanced gene expression of these three enzymes in neuron-derived cells such as N1E-115.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalNeuroscience Letters
Volume238
Issue number1-2
DOIs
Publication statusPublished - 28-11-1997

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Neuroblastoma
Lipopolysaccharides
sepiapterin reductase
Guanosine Triphosphate
Gene Expression
Cell Line
Enzymes
Reverse Transcription
Neurons
Polymerase Chain Reaction
Messenger RNA
DNA
sapropterin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "Effect of lipopolysaccharide on the gene expression of the enzymes involved in tetrahydrobiopterin de novo biosynthesis in murine neuroblastoma cell line N1E-115",
abstract = "The amounts of messenger RNA for three enzymes, namely guanosine triphosphate (GTP) cyclohydrolase I, 6-pyruvoyltetrahydropterin synthase, and sepiapterin reductase, all of which are involved in the de novo biosynthesis of (6R)-L-erythrodihydroxypropyl-2-amino-4-hydroxy-5,6,7,8- tetrahydropteridine (BH4) from GTP, were measured quantitatively in murine neuroblastoma cell line N1E-115 by the competitive polymerase chain reaction (PCR) technique after reverse transcription using a heterologous DNA fragment as an internal standard. Twenty-four hour activation of this cell line with 1 μg/ml lipopolysaccharide resulted in statistically significant increases in the amounts of the messages of all three enzymes. Our data suggest that lipopolysaccharide can activate the intrinsic pathway resulting in the enhanced gene expression of these three enzymes in neuron-derived cells such as N1E-115.",
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Effect of lipopolysaccharide on the gene expression of the enzymes involved in tetrahydrobiopterin de novo biosynthesis in murine neuroblastoma cell line N1E-115. / Mori, Keiji; Nakashima, Akira; Nagatsu, Toshiharu; Ota, Akira.

In: Neuroscience Letters, Vol. 238, No. 1-2, 28.11.1997, p. 21-24.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of lipopolysaccharide on the gene expression of the enzymes involved in tetrahydrobiopterin de novo biosynthesis in murine neuroblastoma cell line N1E-115

AU - Mori, Keiji

AU - Nakashima, Akira

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AU - Ota, Akira

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