TY - JOUR
T1 - Effect of polymyxin B-immobilized fiber hemoperfusion on respiratory impairment, hepatocellular dysfunction, and leucopenia in a neonatal sepsis model
AU - Hussein, Mohamed Hamed
AU - Daoud, Ghada A.
AU - Kakita, Hiroki
AU - Kato, Shin
AU - Goto, Tatenobu
AU - Kamei, Michi
AU - Goto, Kenji
AU - Ozaki, Yasuhiko
AU - Ito, Tetsuya
AU - Fukuda, Sumio
AU - Kato, Ineko
AU - Suzuki, Satoshi
AU - Hashimoto, Takashi
AU - Togari, Hajime
N1 - Funding Information:
Open Access Ver?ffentlichung erm?glicht und organisiert durch Projekt DEAL.
PY - 2010/2
Y1 - 2010/2
N2 - Purpose: Sepsis and septic shock remain a major source of morbidity and mortality in neonates despite advances in antimicrobials and aggressive supportive care. Our aim was to study the effects of polymyxin-B direct hemoperfusion (PMX-DHP) therapy on sepsis-induced respiratory impairment, liver dysfunction and leucopenia in a neonatal cecal ligation and perforation (CLP) model. Methods: Fourteen anesthetized and mechanically ventilated 3-day-old piglets underwent CLP and an arteriovenous extracorporeal circuit from 3 h until 6 h post-CLP, with a PMX column in the PMX-DHP treated group (7 piglets). Changes in oxygen saturation, PCO2, base excess, white blood cell (WBC) count, platelet count, hematocrit (Hct%), serum glutamate pyruvate transaminase (SGPT), and serum glutamic oxaloacetic transaminase were measured before CLP and at 1, 3 and 6 h after. Results: At 6 h, the PMX-DHP group showed lower Hct%, and SGPT in comparison to the control group, but higher oxygen saturation and WBC count. No effects on the platelet count were found. The survival times of the PMX-DHP group were longer than in control. Conclusion: PMX-DHP therapy limited the respiratory impairment, liver dysfunction and leucopenia in a neonatal septic model, which resulted in an improvement of survival time.
AB - Purpose: Sepsis and septic shock remain a major source of morbidity and mortality in neonates despite advances in antimicrobials and aggressive supportive care. Our aim was to study the effects of polymyxin-B direct hemoperfusion (PMX-DHP) therapy on sepsis-induced respiratory impairment, liver dysfunction and leucopenia in a neonatal cecal ligation and perforation (CLP) model. Methods: Fourteen anesthetized and mechanically ventilated 3-day-old piglets underwent CLP and an arteriovenous extracorporeal circuit from 3 h until 6 h post-CLP, with a PMX column in the PMX-DHP treated group (7 piglets). Changes in oxygen saturation, PCO2, base excess, white blood cell (WBC) count, platelet count, hematocrit (Hct%), serum glutamate pyruvate transaminase (SGPT), and serum glutamic oxaloacetic transaminase were measured before CLP and at 1, 3 and 6 h after. Results: At 6 h, the PMX-DHP group showed lower Hct%, and SGPT in comparison to the control group, but higher oxygen saturation and WBC count. No effects on the platelet count were found. The survival times of the PMX-DHP group were longer than in control. Conclusion: PMX-DHP therapy limited the respiratory impairment, liver dysfunction and leucopenia in a neonatal septic model, which resulted in an improvement of survival time.
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U2 - 10.1007/s00383-009-2476-x
DO - 10.1007/s00383-009-2476-x
M3 - Article
C2 - 19802625
AN - SCOPUS:77649232969
SN - 0179-0358
VL - 26
SP - 187
EP - 193
JO - Pediatric Surgery International
JF - Pediatric Surgery International
IS - 2
ER -