Effect of polymyxin B-immobilized fiber hemoperfusion on respiratory impairment, hepatocellular dysfunction, and leucopenia in a neonatal sepsis model

Mohamed Hamed Hussein, Ghada A. Daoud, Hiroki Kakita, Shin Kato, Tatenobu Goto, Michi Kamei, Kenji Goto, Yasuhiko Ozaki, Tetsuya Ito, Sumio Fukuda, Ineko Kato, Satoshi Suzuki, Takashi Hashimoto, Hajime Togari

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Abstract

Purpose: Sepsis and septic shock remain a major source of morbidity and mortality in neonates despite advances in antimicrobials and aggressive supportive care. Our aim was to study the effects of polymyxin-B direct hemoperfusion (PMX-DHP) therapy on sepsis-induced respiratory impairment, liver dysfunction and leucopenia in a neonatal cecal ligation and perforation (CLP) model. Methods: Fourteen anesthetized and mechanically ventilated 3-day-old piglets underwent CLP and an arteriovenous extracorporeal circuit from 3 h until 6 h post-CLP, with a PMX column in the PMX-DHP treated group (7 piglets). Changes in oxygen saturation, PCO2, base excess, white blood cell (WBC) count, platelet count, hematocrit (Hct%), serum glutamate pyruvate transaminase (SGPT), and serum glutamic oxaloacetic transaminase were measured before CLP and at 1, 3 and 6 h after. Results: At 6 h, the PMX-DHP group showed lower Hct%, and SGPT in comparison to the control group, but higher oxygen saturation and WBC count. No effects on the platelet count were found. The survival times of the PMX-DHP group were longer than in control. Conclusion: PMX-DHP therapy limited the respiratory impairment, liver dysfunction and leucopenia in a neonatal septic model, which resulted in an improvement of survival time.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalPediatric Surgery International
Volume26
Issue number2
DOIs
Publication statusPublished - 01-02-2010
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

Hussein, M. H., Daoud, G. A., Kakita, H., Kato, S., Goto, T., Kamei, M., Goto, K., Ozaki, Y., Ito, T., Fukuda, S., Kato, I., Suzuki, S., Hashimoto, T., & Togari, H. (2010). Effect of polymyxin B-immobilized fiber hemoperfusion on respiratory impairment, hepatocellular dysfunction, and leucopenia in a neonatal sepsis model. Pediatric Surgery International, 26(2), 187-193. https://doi.org/10.1007/s00383-009-2476-x