Effect of prostaglandin F on Ca2+ influx in osteoblast‐like cells: Function of tyrosine kinase

Atsushi Suzuki, Osamu Kozawa, Hidehiko Saito, Yutaka Oiso

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18 Citations (Scopus)

Abstract

We previously reported that pertussis toxin–sensitive GTP‐binding protein is involved in prostaglandin F (PGF)‐induced phosphoinositide (PI) hydrolysis in osteoblast‐like MC3T3‐E1 cells [Miwa et al. (1990): Biochem Biophys Res Commun 171:1229–1235]. In the present study, we investigated the mechanism of PGF‐induced Ca2+ influx in MC3T3‐E1 cells. PGF‐induced formation of total inositol phosphates (IPs) was markedly reduced by the depletion of extracellular Ca2+ with EGTA. On the other hand, the depletion of extracellular Ca2+ had little effect on PGF‐induced inositol 1,4,5‐trisphosphate formation. PGF stimulated 45Ca2+ influx dose dependently, attaining a maximum effect at 10 nM. Dose of PGF above 10 nM caused less than maximal stimulation. Genistein, an inhibitor of protein tyrosine kinase, which by itself had little effect on 45Ca2+ influx, significantly suppressed the PGF‐induced 45Ca2+ influx in a dose‐dependent manner in the range between 1 μg/ml and 0.1 mg/ml. Sodium orthovanadate, an inhibitor of protein tyrosine phosphatases, enhanced the PGF‐induced 45Ca2+ influx. Genistein also suppressed the PGF‐induced total IPs formation dose dependently in the range between 1 μg/ml and 0.1 mg/ml. However, it had little effect on the PGF‐induced inositol 1,4,5‐trisphosphate formation. The pretreatment with pertussis toxin had little effect on the PGF‐induced 45Ca2+ influx. These results strongly suggest that PGF stimulates Ca2+ mobilization from extracellular space and PI hydrolysis via independent pathways in osteoblast‐like cells, and the PGF‐induced Ca2+ influx is regulated by protein tyrosine kinase, resulting in the promotion of PI hydrolysis.

Original languageEnglish
Pages (from-to)487-493
Number of pages7
JournalJournal of Cellular Biochemistry
Volume54
Issue number4
DOIs
Publication statusPublished - 04-1994

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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