TY - JOUR
T1 - Effect of retinoic acid on prostaglandin F(2α)-induced phospholipase D activity in osteoblast-like cells
AU - Kozawa, O.
AU - Suzuki, A.
AU - Shinoda, J.
AU - Oiso, Y.
PY - 1996/9
Y1 - 1996/9
N2 - We previously reported that prostaglandin F(2α) (PGF(2α)) activates phosphatidylcholine-hydrolyzing phospholipase D independently from the activation of protein kinase C (PKC) in osteoblast-like MC3T3-E1 cells, and reported that pertussis toxin-sensitive GTP-binding protein (G-protein) is involved in the PGF(2α)-induced phospholipase D activation. In this study, we examined the effect of retinoic acid (RA) on the phospholipase D activity stimulated by PGF(2α) in these cells. The pretreatment of RA markedly inhibited the formation of choline induced by PGF(2α) (10 μM) in a dose-dependent manner in the range between 1 nM and 0.1 μM. This inhibitory effect of RA was dependent on the time of pretreatment up to 8 h. However, RA had little effect on the choline formation induced by NaF, a G-protein activator, or 12-O-tetradecanoylphorbol-13-acetate, an activator of PKC. These results strongly suggest that RA suppresses the phospholipase D activated by PGF(2α) in osteoblast-like cells and that the effect of RA is exerted at the point between PGF(2α) receptor and G-protein.
AB - We previously reported that prostaglandin F(2α) (PGF(2α)) activates phosphatidylcholine-hydrolyzing phospholipase D independently from the activation of protein kinase C (PKC) in osteoblast-like MC3T3-E1 cells, and reported that pertussis toxin-sensitive GTP-binding protein (G-protein) is involved in the PGF(2α)-induced phospholipase D activation. In this study, we examined the effect of retinoic acid (RA) on the phospholipase D activity stimulated by PGF(2α) in these cells. The pretreatment of RA markedly inhibited the formation of choline induced by PGF(2α) (10 μM) in a dose-dependent manner in the range between 1 nM and 0.1 μM. This inhibitory effect of RA was dependent on the time of pretreatment up to 8 h. However, RA had little effect on the choline formation induced by NaF, a G-protein activator, or 12-O-tetradecanoylphorbol-13-acetate, an activator of PKC. These results strongly suggest that RA suppresses the phospholipase D activated by PGF(2α) in osteoblast-like cells and that the effect of RA is exerted at the point between PGF(2α) receptor and G-protein.
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U2 - 10.1016/S0952-3278(96)90090-3
DO - 10.1016/S0952-3278(96)90090-3
M3 - Article
C2 - 8931111
AN - SCOPUS:0029997184
SN - 0952-3278
VL - 55
SP - 151
EP - 154
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
IS - 3
ER -