Effect of tumor necrosis factor or interleukin-1 on muscle amino acid uptake and the role of glucocorticoids

O. Zamir, P. O. Hasselgren, H. James, Takashi Higashiguchi, J. E. Fischer

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Muscle amino acid uptake is inhibited during sepsis and endotoxemia. Cytokines, in particular tumor necrosis factor (TNF) and interleukin-1 (IL- 1), have been implicated as mediators of metabolic alterations in sepsis and other critical illness. In this study, we examined the effect of TNF and IL- 1 on muscle amino acid uptake and tested the hypothesis that cytokine- induced changes in muscle amino acid uptake are mediated by glucocorticoids. Intraperitoneal injection in rats of 100 micrograms per kilogram body weight of human recombinant TNFα (rTNFα) or rIL-1α resulted, two hours later, in 36 and 24 percent reduction, respectively, of amino acid transport in incubated soleus muscles, determined as intracellular uptake of α- aminoisobutyric acid. When rats were treated with the glucocorticoid receptor antagonist RU 38486 (5 milligrams per kilogram of body weight) two hours before cytokine injection, the inhibitory effect on muscle amino acid transport of TNF was blocked, whereas that of IL-1 was unaffected. The present results suggest that TNF and IL-1 may regulate amino acid transport in skeletal muscle and that the effect of TNF, but not that of IL-1, is at least partly mediated by glucocorticoids.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalSurgery Gynecology and Obstetrics
Volume177
Issue number1
Publication statusPublished - 01-01-1993

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Interleukin-1
Glucocorticoids
Tumor Necrosis Factor-alpha
Amino Acids
Muscles
Cytokines
Sepsis
Skeletal Muscle
Body Weight
Aminoisobutyric Acids
Mifepristone
Endotoxemia
Glucocorticoid Receptors
Intraperitoneal Injections
Critical Illness
Injections

All Science Journal Classification (ASJC) codes

  • Surgery
  • Obstetrics and Gynaecology

Cite this

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Effect of tumor necrosis factor or interleukin-1 on muscle amino acid uptake and the role of glucocorticoids. / Zamir, O.; Hasselgren, P. O.; James, H.; Higashiguchi, Takashi; Fischer, J. E.

In: Surgery Gynecology and Obstetrics, Vol. 177, No. 1, 01.01.1993, p. 27-32.

Research output: Contribution to journalArticle

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