Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice

Yohei Ishikawa, Kouhei Hashizume, Seishi Kishimoto, Yu Tezuka, Hideo Nishigori, Naoki Yamamoto, Yoshitaka Kondo, Naoki Maruyama, Akihito Ishigami, Daijiro Kurosaka

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Abstract

We investigated whether decreased vitamin C (VC) in a mouse model increases lens opacity (cataract) induced by invivo exposure to ultraviolet radiation type B (UVR-B).Senescence marker protein-30 (SMP30) knockout (KO) mice, which cannot synthesize VC due to genetic disruption of the gluconolactonase (GNL) gene, were divided into 2 groups: VC sufficient (VC (+)) and VC deficient (VC (-)). Starting at 1 month of age, these groups had free access to water containing 0.0375 and 1.5g/L of VC, respectively. SMP30 KO VC (-), SMP30 KO VC (+), and wild-type (WT) mice, all 14 weeks of age, were unilaterally exposed invivo to UVR-B (200mW/cm 2) for 100s twice a week for 3 weeks (total: 1200mJ/cm 2). At 48h after the last UVR-B exposure, cataract morphology was documented, and the ratio of cataract induction was quantified as the cataract area ratio (opacity area/anterior capsule).UVR-B exposure induced cataract mainly at anterior sub-capsular in SMP30 KO VC (-), SMP30 KO VC (+), and WT mice. In SMP30 KO VC (-) lenses the opacities were more extensive than in SMP30 KO VC (+) or WT lenses (cataract area ratios: 59.3%±10% vs. 32.2%±11.7% or 29.0%±9.0%; P<0.01).In conclusion, VC depletion may increase the susceptibility to develop UVR-B induced cataracts in mice unable to endogenously produce VC.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalExperimental Eye Research
Volume94
Issue number1
DOIs
Publication statusPublished - 01-01-2012

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gluconolactonase
Knockout Mice
Cataract
Ascorbic Acid
Radiation
Proteins

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ishikawa, Y., Hashizume, K., Kishimoto, S., Tezuka, Y., Nishigori, H., Yamamoto, N., ... Kurosaka, D. (2012). Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice. Experimental Eye Research, 94(1), 85-89. https://doi.org/10.1016/j.exer.2011.11.010
Ishikawa, Yohei ; Hashizume, Kouhei ; Kishimoto, Seishi ; Tezuka, Yu ; Nishigori, Hideo ; Yamamoto, Naoki ; Kondo, Yoshitaka ; Maruyama, Naoki ; Ishigami, Akihito ; Kurosaka, Daijiro. / Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice. In: Experimental Eye Research. 2012 ; Vol. 94, No. 1. pp. 85-89.
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abstract = "We investigated whether decreased vitamin C (VC) in a mouse model increases lens opacity (cataract) induced by invivo exposure to ultraviolet radiation type B (UVR-B).Senescence marker protein-30 (SMP30) knockout (KO) mice, which cannot synthesize VC due to genetic disruption of the gluconolactonase (GNL) gene, were divided into 2 groups: VC sufficient (VC (+)) and VC deficient (VC (-)). Starting at 1 month of age, these groups had free access to water containing 0.0375 and 1.5g/L of VC, respectively. SMP30 KO VC (-), SMP30 KO VC (+), and wild-type (WT) mice, all 14 weeks of age, were unilaterally exposed invivo to UVR-B (200mW/cm 2) for 100s twice a week for 3 weeks (total: 1200mJ/cm 2). At 48h after the last UVR-B exposure, cataract morphology was documented, and the ratio of cataract induction was quantified as the cataract area ratio (opacity area/anterior capsule).UVR-B exposure induced cataract mainly at anterior sub-capsular in SMP30 KO VC (-), SMP30 KO VC (+), and WT mice. In SMP30 KO VC (-) lenses the opacities were more extensive than in SMP30 KO VC (+) or WT lenses (cataract area ratios: 59.3{\%}±10{\%} vs. 32.2{\%}±11.7{\%} or 29.0{\%}±9.0{\%}; P<0.01).In conclusion, VC depletion may increase the susceptibility to develop UVR-B induced cataracts in mice unable to endogenously produce VC.",
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Ishikawa, Y, Hashizume, K, Kishimoto, S, Tezuka, Y, Nishigori, H, Yamamoto, N, Kondo, Y, Maruyama, N, Ishigami, A & Kurosaka, D 2012, 'Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice', Experimental Eye Research, vol. 94, no. 1, pp. 85-89. https://doi.org/10.1016/j.exer.2011.11.010

Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice. / Ishikawa, Yohei; Hashizume, Kouhei; Kishimoto, Seishi; Tezuka, Yu; Nishigori, Hideo; Yamamoto, Naoki; Kondo, Yoshitaka; Maruyama, Naoki; Ishigami, Akihito; Kurosaka, Daijiro.

In: Experimental Eye Research, Vol. 94, No. 1, 01.01.2012, p. 85-89.

Research output: Contribution to journalArticle

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T1 - Effect of vitamin C depletion on UVR-B induced cataract in SMP30/GNL knockout mice

AU - Ishikawa, Yohei

AU - Hashizume, Kouhei

AU - Kishimoto, Seishi

AU - Tezuka, Yu

AU - Nishigori, Hideo

AU - Yamamoto, Naoki

AU - Kondo, Yoshitaka

AU - Maruyama, Naoki

AU - Ishigami, Akihito

AU - Kurosaka, Daijiro

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We investigated whether decreased vitamin C (VC) in a mouse model increases lens opacity (cataract) induced by invivo exposure to ultraviolet radiation type B (UVR-B).Senescence marker protein-30 (SMP30) knockout (KO) mice, which cannot synthesize VC due to genetic disruption of the gluconolactonase (GNL) gene, were divided into 2 groups: VC sufficient (VC (+)) and VC deficient (VC (-)). Starting at 1 month of age, these groups had free access to water containing 0.0375 and 1.5g/L of VC, respectively. SMP30 KO VC (-), SMP30 KO VC (+), and wild-type (WT) mice, all 14 weeks of age, were unilaterally exposed invivo to UVR-B (200mW/cm 2) for 100s twice a week for 3 weeks (total: 1200mJ/cm 2). At 48h after the last UVR-B exposure, cataract morphology was documented, and the ratio of cataract induction was quantified as the cataract area ratio (opacity area/anterior capsule).UVR-B exposure induced cataract mainly at anterior sub-capsular in SMP30 KO VC (-), SMP30 KO VC (+), and WT mice. In SMP30 KO VC (-) lenses the opacities were more extensive than in SMP30 KO VC (+) or WT lenses (cataract area ratios: 59.3%±10% vs. 32.2%±11.7% or 29.0%±9.0%; P<0.01).In conclusion, VC depletion may increase the susceptibility to develop UVR-B induced cataracts in mice unable to endogenously produce VC.

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