Effect of vitamin D 3 on prostaglandin E 2 synthesis in osteoblast-like cells

Atsushi Suzuki, H. Tokuda, J. Kotoyori, Y. Ito, Y. Oiso, O. Kozawa

Research output: Contribution to journalArticle

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Abstract

We previously showed that prostaglandin (PG) F stimulates PGE 2 synthesis in osteoblast-like MC3T3-E1 cells, and that the activation of protein kinase C (PKC) amplifies the effect of PGF through the potentiation of phospholipase A 2 activity (H. Tokuda, Y. Oiso and O. Kozawa, J Cell Biochem. 48: 262-268, 1992). In the present study, we investigated the effects of vitamin D 3 on PGF -induced PGE 2 synthesis in MC3T3-E1 cells. The pretreatment with 1,25-dihydroxyvitamin D 3 [1,25-(OH) 2 D 3 ], an active form of vitamin D 3 , significantly inhibited the PGF -induced PGE 2 synthesis in a dose-dependent manner in the range between 1 pM and 1 nM. This inhibitory effect of 1,25-(OH) 2 D 3 was dependent on the time of pretreatment up to 8 h. On the contrary, the pretreatment with 24,25-dihydroxyvitamin D 3 , an inactive form of vitamin D 3 , had little effect on the PGF 2 α-induced PGE 2 synthesis in these cells. However, the pretreatment with 1,25-(OH) 2 D 3 no longer affected the amplification of PGF -induced PGE 2 synthesis by 12-O-tetradecanoylphorbol-13-acetate, a PKC activator. These results strongly suggest that 1,25-(OH) 2 D 3 inhibits PGF -induced PGE 2 synthesis in osteoblast-like cells, however, the activation of PKC reverses this inhibitory effect of 1,25-(OH) 2 D 3 .

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume51
Issue number1
DOIs
Publication statusPublished - 01-01-1994
Externally publishedYes

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Cholecalciferol
Osteoblasts
Prostaglandins F
Prostaglandins E
Protein Kinase C
24,25-Dihydroxyvitamin D 3
Chemical activation
Phospholipases A
Tetradecanoylphorbol Acetate
Amplification
Acetates

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

Cite this

Suzuki, Atsushi ; Tokuda, H. ; Kotoyori, J. ; Ito, Y. ; Oiso, Y. ; Kozawa, O. / Effect of vitamin D 3 on prostaglandin E 2 synthesis in osteoblast-like cells In: Prostaglandins, Leukotrienes and Essential Fatty Acids. 1994 ; Vol. 51, No. 1. pp. 27-31.
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abstract = "We previously showed that prostaglandin (PG) F 2α stimulates PGE 2 synthesis in osteoblast-like MC3T3-E1 cells, and that the activation of protein kinase C (PKC) amplifies the effect of PGF 2α through the potentiation of phospholipase A 2 activity (H. Tokuda, Y. Oiso and O. Kozawa, J Cell Biochem. 48: 262-268, 1992). In the present study, we investigated the effects of vitamin D 3 on PGF 2α -induced PGE 2 synthesis in MC3T3-E1 cells. The pretreatment with 1,25-dihydroxyvitamin D 3 [1,25-(OH) 2 D 3 ], an active form of vitamin D 3 , significantly inhibited the PGF 2α -induced PGE 2 synthesis in a dose-dependent manner in the range between 1 pM and 1 nM. This inhibitory effect of 1,25-(OH) 2 D 3 was dependent on the time of pretreatment up to 8 h. On the contrary, the pretreatment with 24,25-dihydroxyvitamin D 3 , an inactive form of vitamin D 3 , had little effect on the PGF 2 α-induced PGE 2 synthesis in these cells. However, the pretreatment with 1,25-(OH) 2 D 3 no longer affected the amplification of PGF 2α -induced PGE 2 synthesis by 12-O-tetradecanoylphorbol-13-acetate, a PKC activator. These results strongly suggest that 1,25-(OH) 2 D 3 inhibits PGF 2α -induced PGE 2 synthesis in osteoblast-like cells, however, the activation of PKC reverses this inhibitory effect of 1,25-(OH) 2 D 3 .",
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Effect of vitamin D 3 on prostaglandin E 2 synthesis in osteoblast-like cells . / Suzuki, Atsushi; Tokuda, H.; Kotoyori, J.; Ito, Y.; Oiso, Y.; Kozawa, O.

In: Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 51, No. 1, 01.01.1994, p. 27-31.

Research output: Contribution to journalArticle

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T1 - Effect of vitamin D 3 on prostaglandin E 2 synthesis in osteoblast-like cells

AU - Suzuki, Atsushi

AU - Tokuda, H.

AU - Kotoyori, J.

AU - Ito, Y.

AU - Oiso, Y.

AU - Kozawa, O.

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Y1 - 1994/1/1

N2 - We previously showed that prostaglandin (PG) F 2α stimulates PGE 2 synthesis in osteoblast-like MC3T3-E1 cells, and that the activation of protein kinase C (PKC) amplifies the effect of PGF 2α through the potentiation of phospholipase A 2 activity (H. Tokuda, Y. Oiso and O. Kozawa, J Cell Biochem. 48: 262-268, 1992). In the present study, we investigated the effects of vitamin D 3 on PGF 2α -induced PGE 2 synthesis in MC3T3-E1 cells. The pretreatment with 1,25-dihydroxyvitamin D 3 [1,25-(OH) 2 D 3 ], an active form of vitamin D 3 , significantly inhibited the PGF 2α -induced PGE 2 synthesis in a dose-dependent manner in the range between 1 pM and 1 nM. This inhibitory effect of 1,25-(OH) 2 D 3 was dependent on the time of pretreatment up to 8 h. On the contrary, the pretreatment with 24,25-dihydroxyvitamin D 3 , an inactive form of vitamin D 3 , had little effect on the PGF 2 α-induced PGE 2 synthesis in these cells. However, the pretreatment with 1,25-(OH) 2 D 3 no longer affected the amplification of PGF 2α -induced PGE 2 synthesis by 12-O-tetradecanoylphorbol-13-acetate, a PKC activator. These results strongly suggest that 1,25-(OH) 2 D 3 inhibits PGF 2α -induced PGE 2 synthesis in osteoblast-like cells, however, the activation of PKC reverses this inhibitory effect of 1,25-(OH) 2 D 3 .

AB - We previously showed that prostaglandin (PG) F 2α stimulates PGE 2 synthesis in osteoblast-like MC3T3-E1 cells, and that the activation of protein kinase C (PKC) amplifies the effect of PGF 2α through the potentiation of phospholipase A 2 activity (H. Tokuda, Y. Oiso and O. Kozawa, J Cell Biochem. 48: 262-268, 1992). In the present study, we investigated the effects of vitamin D 3 on PGF 2α -induced PGE 2 synthesis in MC3T3-E1 cells. The pretreatment with 1,25-dihydroxyvitamin D 3 [1,25-(OH) 2 D 3 ], an active form of vitamin D 3 , significantly inhibited the PGF 2α -induced PGE 2 synthesis in a dose-dependent manner in the range between 1 pM and 1 nM. This inhibitory effect of 1,25-(OH) 2 D 3 was dependent on the time of pretreatment up to 8 h. On the contrary, the pretreatment with 24,25-dihydroxyvitamin D 3 , an inactive form of vitamin D 3 , had little effect on the PGF 2 α-induced PGE 2 synthesis in these cells. However, the pretreatment with 1,25-(OH) 2 D 3 no longer affected the amplification of PGF 2α -induced PGE 2 synthesis by 12-O-tetradecanoylphorbol-13-acetate, a PKC activator. These results strongly suggest that 1,25-(OH) 2 D 3 inhibits PGF 2α -induced PGE 2 synthesis in osteoblast-like cells, however, the activation of PKC reverses this inhibitory effect of 1,25-(OH) 2 D 3 .

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