Effects of aloe-emodin and emodin on proliferation of the MKN45 human gastric cancer cell line

Takeshi Chihara, Kan Shimpo, Hidehiko Beppu, Naoki Yamamoto, Takaaki Kaneko, Kazumasa Wakamatsu, Shigeru Sonoda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone; AE) and emodin (1,3,8-trihydroxy-6-methylanthraquinone; EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines; however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.

Original languageEnglish
Pages (from-to)3887-3891
Number of pages5
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number9
DOIs
Publication statusPublished - 01-01-2015

Fingerprint

Emodin
Stomach Neoplasms
Anthraquinones
Cell Line
Cell Death
Neoplasms
Cell Cycle Resting Phase
G2 Phase
Polyamines
G1 Phase
DNA Fragmentation
Cell Division
Hydroxyl Radical
Cell Cycle
Cell Proliferation
Growth
aloe emodin

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Chihara, Takeshi ; Shimpo, Kan ; Beppu, Hidehiko ; Yamamoto, Naoki ; Kaneko, Takaaki ; Wakamatsu, Kazumasa ; Sonoda, Shigeru. / Effects of aloe-emodin and emodin on proliferation of the MKN45 human gastric cancer cell line. In: Asian Pacific Journal of Cancer Prevention. 2015 ; Vol. 16, No. 9. pp. 3887-3891.
@article{05ca8aaba73c4f2ab6221fe9d6e5bd24,
title = "Effects of aloe-emodin and emodin on proliferation of the MKN45 human gastric cancer cell line",
abstract = "Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone; AE) and emodin (1,3,8-trihydroxy-6-methylanthraquinone; EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines; however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.",
author = "Takeshi Chihara and Kan Shimpo and Hidehiko Beppu and Naoki Yamamoto and Takaaki Kaneko and Kazumasa Wakamatsu and Shigeru Sonoda",
year = "2015",
month = "1",
day = "1",
doi = "10.7314/APJCP.2015.16.9.3887",
language = "English",
volume = "16",
pages = "3887--3891",
journal = "Asian Pacific Journal of Cancer Prevention",
issn = "1513-7368",
publisher = "Asian Pacific Organization for Cancer Prevention",
number = "9",

}

Effects of aloe-emodin and emodin on proliferation of the MKN45 human gastric cancer cell line. / Chihara, Takeshi; Shimpo, Kan; Beppu, Hidehiko; Yamamoto, Naoki; Kaneko, Takaaki; Wakamatsu, Kazumasa; Sonoda, Shigeru.

In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 9, 01.01.2015, p. 3887-3891.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of aloe-emodin and emodin on proliferation of the MKN45 human gastric cancer cell line

AU - Chihara, Takeshi

AU - Shimpo, Kan

AU - Beppu, Hidehiko

AU - Yamamoto, Naoki

AU - Kaneko, Takaaki

AU - Wakamatsu, Kazumasa

AU - Sonoda, Shigeru

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone; AE) and emodin (1,3,8-trihydroxy-6-methylanthraquinone; EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines; however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.

AB - Aloe-emodin (1, 8-dihydroxy-3-hydroxyl-methylanthraquinone; AE) and emodin (1,3,8-trihydroxy-6-methylanthraquinone; EM) are anthraquinone derivatives that have been detected in some medical plants and share similar anthraquinone structures. AE and EM have been shown to exhibit anticancer activities in various cancer cell lines; however, the inhibitory effects of these derivatives on the growth of cancer cells were previously reported to be different. Gastric cancer is the second most common cause of cancer cell death worldwide. In the present study, we examined the inhibitory effects of 0.05 mM AE and 0.05 mM EM on the proliferation of the MKN45 human gastric cancer cell line. The proliferation of MKN45 cells was significantly inhibited in AE- and EM-treated groups 24 h and 48 h after treatment. Furthermore, the inhibitory effects of EM were stronger than those of AE. The cell cycle of MKN45 cells were arrested in G0/G1 phase or G0/G1 and G2/M phases by AE and EM, respectively. However, an analysis of intracellular polyamine levels and DNA fragmentation revealed that the mechanisms underlying cell death following cell arrest induced by AE and EM differed.

UR - http://www.scopus.com/inward/record.url?scp=84930165041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930165041&partnerID=8YFLogxK

U2 - 10.7314/APJCP.2015.16.9.3887

DO - 10.7314/APJCP.2015.16.9.3887

M3 - Article

VL - 16

SP - 3887

EP - 3891

JO - Asian Pacific Journal of Cancer Prevention

JF - Asian Pacific Journal of Cancer Prevention

SN - 1513-7368

IS - 9

ER -