Effects of Carbon-Ion Beams on Human Pancreatic Cancer Cell Lines That Differ in Genetic Status

Yoshifumi Matsui, Takehide Asano, Takashi Kenmochi, Mayumi Iwakawa, Takashi Imai, Takenori Ochiai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The relative biologic effectiveness (RBE) of carbon-ion beams at 3 different linear energy transfer (LET) values (13, 50, and 80 keV/μm) accelerated by the Heavy Ion Medical Accelerator in Chiba on human pancreatic cancer cell lines differing in genetic status was determined. The RBE values were calculated as D10, the dose (Gy) required to reduce the surviving fraction to 10%, relative to X-rays. We also investigated apoptosis and the relationship between D10 and the cell cycle checkpoint using morphologic examination and flow cytometry analysis, respectively. The RBE values calculated by the D10 values ranged from 1.16 to 1.77 for the 13-keV/μm beam and from 1.83 to 2.46 for the 80-keV/μm beam. A correlation between the D10 values of each cell line and intensity of G2/M arrest was observed. In contrast, LET values did not clearly correlate with induction of apoptosis. These results suggest that carbon-ion beam therapy is a promising modality. Elucidation of the mechanisms of G2/M arrest and apoptosis may provide clues to enhancing the effects of radiation on pancreatic cancer.

Original languageEnglish
Pages (from-to)24-28
Number of pages5
JournalAmerican Journal of Clinical Oncology: Cancer Clinical Trials
Volume27
Issue number1
DOIs
Publication statusPublished - 01-02-2004

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Relative Biological Effectiveness
Pancreatic Neoplasms
Linear Energy Transfer
Carbon
Ions
Apoptosis
Cell Line
Heavy Ion Radiotherapy
Heavy Ions
Radiation Effects
Cell Cycle Checkpoints
Flow Cytometry
X-Rays

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Matsui, Yoshifumi ; Asano, Takehide ; Kenmochi, Takashi ; Iwakawa, Mayumi ; Imai, Takashi ; Ochiai, Takenori. / Effects of Carbon-Ion Beams on Human Pancreatic Cancer Cell Lines That Differ in Genetic Status. In: American Journal of Clinical Oncology: Cancer Clinical Trials. 2004 ; Vol. 27, No. 1. pp. 24-28.
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Effects of Carbon-Ion Beams on Human Pancreatic Cancer Cell Lines That Differ in Genetic Status. / Matsui, Yoshifumi; Asano, Takehide; Kenmochi, Takashi; Iwakawa, Mayumi; Imai, Takashi; Ochiai, Takenori.

In: American Journal of Clinical Oncology: Cancer Clinical Trials, Vol. 27, No. 1, 01.02.2004, p. 24-28.

Research output: Contribution to journalArticle

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