TY - JOUR
T1 - Effects of Chronic Vardenafil Treatment Persist after End of Treatment in Rats with Acute Arteriogenic Erectile Dysfunction
AU - Hotta, Yuji
AU - Ohno, Risa
AU - Kataoka, Tomoya
AU - Mikumo, Mayumi
AU - Takahata, Yu
AU - Ohno, Masae
AU - Maeda, Yasuhiro
AU - Kimura, Kazunori
N1 - Funding Information:
The authors are grateful to Bayer for providing vardenafil. This study was supported by a Grant‐in‐Aid for Scientific Research (C) (No. 19590153) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2012/7
Y1 - 2012/7
N2 - Introduction. In our previous study, chronic vardenafil treatment improved erectile function soon after the end of the treatment in rats with acute arteriogenic erectile dysfunction (ED). Aim. The aim of this study is to evaluate whether the effects of chronic vardenafil treatment persist after the end of treatment using rats with acute arteriogenic ED. Methods. Rats were randomly divided into three groups: (i) control; (ii) ligation; and (iii) vardenafil+no treatment. Rats in the ligation and vardenafil+no treatment groups underwent ligature of the bilateral internal iliac arteries to induce acute arteriogenic ED and were subsequently treated with vehicle or vardenafil (4.0mg/kg/day), respectively, for 3 weeks. Subsequently, all rats were kept for a further 2 weeks with no treatment. Rats in the control group underwent sham surgery. Main Outcome Measures. Erectile function was assessed by changes in intracavernous pressure (ICP). Smooth muscle (SM)/collagen ratios in corpus cavernosum were analyzed by Masson trichrome staining. Transforming growth factor-β1 (TGF-β1) mRNA and protein levels in corpus cavernosum (CC) were, respectively, evaluated by real-time polymerase chain reaction (PCR) analysis and Western blotting analysis. Results. ICP/mean arterial pressure (MAP) in the ligation group remained significantly lower than that in control group (P<0.01). Despite no treatment for 2 weeks, ICP/MAP in the var+no treatment group remained significantly higher than that in ligation group (P<0.05). SM/collagen ratio in the ligation group remained significantly lower when compared with the control group (P<0.01). The ratio in the var+no treatment group remained significantly higher when compared with the ligation group at 2 weeks after the end of treatment (P<0.05). TGF-β1 mRNA and protein levels did not differ among the groups. Conclusions. The effects of chronic vardenafil treatment on erectile function and penile structure persist, even after the end of treatment, in acute arteriogenic ED rats.
AB - Introduction. In our previous study, chronic vardenafil treatment improved erectile function soon after the end of the treatment in rats with acute arteriogenic erectile dysfunction (ED). Aim. The aim of this study is to evaluate whether the effects of chronic vardenafil treatment persist after the end of treatment using rats with acute arteriogenic ED. Methods. Rats were randomly divided into three groups: (i) control; (ii) ligation; and (iii) vardenafil+no treatment. Rats in the ligation and vardenafil+no treatment groups underwent ligature of the bilateral internal iliac arteries to induce acute arteriogenic ED and were subsequently treated with vehicle or vardenafil (4.0mg/kg/day), respectively, for 3 weeks. Subsequently, all rats were kept for a further 2 weeks with no treatment. Rats in the control group underwent sham surgery. Main Outcome Measures. Erectile function was assessed by changes in intracavernous pressure (ICP). Smooth muscle (SM)/collagen ratios in corpus cavernosum were analyzed by Masson trichrome staining. Transforming growth factor-β1 (TGF-β1) mRNA and protein levels in corpus cavernosum (CC) were, respectively, evaluated by real-time polymerase chain reaction (PCR) analysis and Western blotting analysis. Results. ICP/mean arterial pressure (MAP) in the ligation group remained significantly lower than that in control group (P<0.01). Despite no treatment for 2 weeks, ICP/MAP in the var+no treatment group remained significantly higher than that in ligation group (P<0.05). SM/collagen ratio in the ligation group remained significantly lower when compared with the control group (P<0.01). The ratio in the var+no treatment group remained significantly higher when compared with the ligation group at 2 weeks after the end of treatment (P<0.05). TGF-β1 mRNA and protein levels did not differ among the groups. Conclusions. The effects of chronic vardenafil treatment on erectile function and penile structure persist, even after the end of treatment, in acute arteriogenic ED rats.
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U2 - 10.1111/j.1743-6109.2012.02742.x
DO - 10.1111/j.1743-6109.2012.02742.x
M3 - Article
C2 - 22548870
AN - SCOPUS:84863525111
SN - 1743-6095
VL - 9
SP - 1782
EP - 1788
JO - Journal of Sexual Medicine
JF - Journal of Sexual Medicine
IS - 7
ER -
