Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain

Sahabudeen Sheik Mohideen, Sahoko Ichihara, Kaviarasan Subramanian, Zhenlie Huang, Hisao Naito, Junzoh Kitoh, Gaku Ichihara

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Abstract

Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain: Sahabudeen Sheik MOHIDEEN, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya University-Objectives: Human cases of 1-bromopropane (1-BP) toxicity showed ataxic gait and cognitive dysfunction, whereas rat studies showed pyknotic shrinkage in cerebellar Purkinje cells and electrophysiological changes in the hippocampus. The present study investigated the effects of 1-BP on astrocytes and oligodendrocytes in the rat cerebellum and hippocampus to find sensitive markers of central nervous system toxicity. Methods: Forty-eight F344 rats were divided into four equal groups and exposed to 1-BP at 0, 400, 800 and 1,000 ppm for 8 h/day; 7 days/week, for 4 weeks. Nine and three rats per group were used for biochemical and histopathological studies, respectively. Results: Kluver-Barrera staining showed pyknotic shrinkage in the cytoplasm of Purkinje cells and nuclei of granular cells in the cerebellum at 1,000 ppm. Immunohistochemical analysis showed increased length of glial fibrillary acidic protein (GFAP)-positive processes of astrocytes in the cerebellum, hippocampus and dentate gyrus at 800 and 1,000 ppm. The myelin basic protein (MBP) level was lower at 1,000 ppm. The numbers of astrocytes and granular cells per tissue volume increased at 400 ppm or higher. Conclusion: The present study showed that elongation of processes of astrocytes accompanies degeneration of granular cells and Purkinje cells in the cerebellum of the rats exposed to 1-BP. The decrease in MBP and number of oligodendrocytes suggest adverse effects on myelination. The increase in astrocyte population per tissue Oligodendrocytesvolume in the cerebellum might be a sensitive marker of 1-BP neurotoxicity, but the underlying mechanism for this change remains elusive.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalJournal of Occupational Health
Volume55
Issue number1
DOIs
Publication statusPublished - 01-01-2013

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Oligodendroglia
Astrocytes
Cerebellum
Purkinje Cells
Brain
Hippocampus
Myelin Basic Protein
Environmental Health
Glial Fibrillary Acidic Protein
Dentate Gyrus
Inbred F344 Rats
Occupational Health
Cell Nucleus
Gait
1-bromopropane
Cytoplasm
Central Nervous System
Medicine
Staining and Labeling
Population

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

Cite this

Mohideen, S. S., Ichihara, S., Subramanian, K., Huang, Z., Naito, H., Kitoh, J., & Ichihara, G. (2013). Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain. Journal of Occupational Health, 55(1), 29-38. https://doi.org/10.1539/joh.12-0118-OA
Mohideen, Sahabudeen Sheik ; Ichihara, Sahoko ; Subramanian, Kaviarasan ; Huang, Zhenlie ; Naito, Hisao ; Kitoh, Junzoh ; Ichihara, Gaku. / Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain. In: Journal of Occupational Health. 2013 ; Vol. 55, No. 1. pp. 29-38.
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abstract = "Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain: Sahabudeen Sheik MOHIDEEN, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya University-Objectives: Human cases of 1-bromopropane (1-BP) toxicity showed ataxic gait and cognitive dysfunction, whereas rat studies showed pyknotic shrinkage in cerebellar Purkinje cells and electrophysiological changes in the hippocampus. The present study investigated the effects of 1-BP on astrocytes and oligodendrocytes in the rat cerebellum and hippocampus to find sensitive markers of central nervous system toxicity. Methods: Forty-eight F344 rats were divided into four equal groups and exposed to 1-BP at 0, 400, 800 and 1,000 ppm for 8 h/day; 7 days/week, for 4 weeks. Nine and three rats per group were used for biochemical and histopathological studies, respectively. Results: Kluver-Barrera staining showed pyknotic shrinkage in the cytoplasm of Purkinje cells and nuclei of granular cells in the cerebellum at 1,000 ppm. Immunohistochemical analysis showed increased length of glial fibrillary acidic protein (GFAP)-positive processes of astrocytes in the cerebellum, hippocampus and dentate gyrus at 800 and 1,000 ppm. The myelin basic protein (MBP) level was lower at 1,000 ppm. The numbers of astrocytes and granular cells per tissue volume increased at 400 ppm or higher. Conclusion: The present study showed that elongation of processes of astrocytes accompanies degeneration of granular cells and Purkinje cells in the cerebellum of the rats exposed to 1-BP. The decrease in MBP and number of oligodendrocytes suggest adverse effects on myelination. The increase in astrocyte population per tissue Oligodendrocytesvolume in the cerebellum might be a sensitive marker of 1-BP neurotoxicity, but the underlying mechanism for this change remains elusive.",
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Mohideen, SS, Ichihara, S, Subramanian, K, Huang, Z, Naito, H, Kitoh, J & Ichihara, G 2013, 'Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain', Journal of Occupational Health, vol. 55, no. 1, pp. 29-38. https://doi.org/10.1539/joh.12-0118-OA

Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain. / Mohideen, Sahabudeen Sheik; Ichihara, Sahoko; Subramanian, Kaviarasan; Huang, Zhenlie; Naito, Hisao; Kitoh, Junzoh; Ichihara, Gaku.

In: Journal of Occupational Health, Vol. 55, No. 1, 01.01.2013, p. 29-38.

Research output: Contribution to journalArticle

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T1 - Effects of exposure to 1-bromopropane on astrocytes and oligodendrocytes in rat brain

AU - Mohideen, Sahabudeen Sheik

AU - Ichihara, Sahoko

AU - Subramanian, Kaviarasan

AU - Huang, Zhenlie

AU - Naito, Hisao

AU - Kitoh, Junzoh

AU - Ichihara, Gaku

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N2 - Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain: Sahabudeen Sheik MOHIDEEN, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya University-Objectives: Human cases of 1-bromopropane (1-BP) toxicity showed ataxic gait and cognitive dysfunction, whereas rat studies showed pyknotic shrinkage in cerebellar Purkinje cells and electrophysiological changes in the hippocampus. The present study investigated the effects of 1-BP on astrocytes and oligodendrocytes in the rat cerebellum and hippocampus to find sensitive markers of central nervous system toxicity. Methods: Forty-eight F344 rats were divided into four equal groups and exposed to 1-BP at 0, 400, 800 and 1,000 ppm for 8 h/day; 7 days/week, for 4 weeks. Nine and three rats per group were used for biochemical and histopathological studies, respectively. Results: Kluver-Barrera staining showed pyknotic shrinkage in the cytoplasm of Purkinje cells and nuclei of granular cells in the cerebellum at 1,000 ppm. Immunohistochemical analysis showed increased length of glial fibrillary acidic protein (GFAP)-positive processes of astrocytes in the cerebellum, hippocampus and dentate gyrus at 800 and 1,000 ppm. The myelin basic protein (MBP) level was lower at 1,000 ppm. The numbers of astrocytes and granular cells per tissue volume increased at 400 ppm or higher. Conclusion: The present study showed that elongation of processes of astrocytes accompanies degeneration of granular cells and Purkinje cells in the cerebellum of the rats exposed to 1-BP. The decrease in MBP and number of oligodendrocytes suggest adverse effects on myelination. The increase in astrocyte population per tissue Oligodendrocytesvolume in the cerebellum might be a sensitive marker of 1-BP neurotoxicity, but the underlying mechanism for this change remains elusive.

AB - Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain: Sahabudeen Sheik MOHIDEEN, et al. Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine, Nagoya University-Objectives: Human cases of 1-bromopropane (1-BP) toxicity showed ataxic gait and cognitive dysfunction, whereas rat studies showed pyknotic shrinkage in cerebellar Purkinje cells and electrophysiological changes in the hippocampus. The present study investigated the effects of 1-BP on astrocytes and oligodendrocytes in the rat cerebellum and hippocampus to find sensitive markers of central nervous system toxicity. Methods: Forty-eight F344 rats were divided into four equal groups and exposed to 1-BP at 0, 400, 800 and 1,000 ppm for 8 h/day; 7 days/week, for 4 weeks. Nine and three rats per group were used for biochemical and histopathological studies, respectively. Results: Kluver-Barrera staining showed pyknotic shrinkage in the cytoplasm of Purkinje cells and nuclei of granular cells in the cerebellum at 1,000 ppm. Immunohistochemical analysis showed increased length of glial fibrillary acidic protein (GFAP)-positive processes of astrocytes in the cerebellum, hippocampus and dentate gyrus at 800 and 1,000 ppm. The myelin basic protein (MBP) level was lower at 1,000 ppm. The numbers of astrocytes and granular cells per tissue volume increased at 400 ppm or higher. Conclusion: The present study showed that elongation of processes of astrocytes accompanies degeneration of granular cells and Purkinje cells in the cerebellum of the rats exposed to 1-BP. The decrease in MBP and number of oligodendrocytes suggest adverse effects on myelination. The increase in astrocyte population per tissue Oligodendrocytesvolume in the cerebellum might be a sensitive marker of 1-BP neurotoxicity, but the underlying mechanism for this change remains elusive.

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