Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2): Changes in GABAA and Benzodiazepine Receptor Binding

Tetsuo Hayashi, Kiyofumi Yamada, Takaaki Hasegawa, Toshitaka Nabeshima, Seiichi Ishihara, Tsutomu Kameyama, Tadaomi Morimasa, Takao Kaneyuki, Toshikiyo Shohmori

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Abstract

We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]fhmitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalThe Japanese Journal of Pharmacology
Volume66
Issue number1
DOIs
Publication statusPublished - 01-01-1994

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Neurotransmitter Receptor
Chinese Traditional Medicine
GABA-A Receptors
Autoradiography
Muscimol
Flunitrazepam
Brain
Anti-Anxiety Agents
kamikihi-to
In Vitro Techniques
Putamen
Thalamus
Anxiety
Binding Sites

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Hayashi, Tetsuo ; Yamada, Kiyofumi ; Hasegawa, Takaaki ; Nabeshima, Toshitaka ; Ishihara, Seiichi ; Kameyama, Tsutomu ; Morimasa, Tadaomi ; Kaneyuki, Takao ; Shohmori, Toshikiyo. / Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2) : Changes in GABAA and Benzodiazepine Receptor Binding. In: The Japanese Journal of Pharmacology. 1994 ; Vol. 66, No. 1. pp. 53-58.
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abstract = "We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]fhmitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.",
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Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2) : Changes in GABAA and Benzodiazepine Receptor Binding. / Hayashi, Tetsuo; Yamada, Kiyofumi; Hasegawa, Takaaki; Nabeshima, Toshitaka; Ishihara, Seiichi; Kameyama, Tsutomu; Morimasa, Tadaomi; Kaneyuki, Takao; Shohmori, Toshikiyo.

In: The Japanese Journal of Pharmacology, Vol. 66, No. 1, 01.01.1994, p. 53-58.

Research output: Contribution to journalArticle

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AU - Hayashi, Tetsuo

AU - Yamada, Kiyofumi

AU - Hasegawa, Takaaki

AU - Nabeshima, Toshitaka

AU - Ishihara, Seiichi

AU - Kameyama, Tsutomu

AU - Morimasa, Tadaomi

AU - Kaneyuki, Takao

AU - Shohmori, Toshikiyo

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N2 - We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]fhmitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.

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