Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2)

Changes in GABAA and Benzodiazepine Receptor Binding

Tetsuo Hayashi, Kiyofumi Yamada, Takaaki Hasegawa, Toshitaka Nabeshima, Seiichi Ishihara, Tsutomu Kameyama, Tadaomi Morimasa, Takao Kaneyuki, Toshikiyo Shohmori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]fhmitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalThe Japanese Journal of Pharmacology
Volume66
Issue number1
DOIs
Publication statusPublished - 01-01-1994
Externally publishedYes

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Neurotransmitter Receptor
Chinese Traditional Medicine
GABA-A Receptors
Autoradiography
Muscimol
Flunitrazepam
Brain
Anti-Anxiety Agents
kamikihi-to
In Vitro Techniques
Putamen
Thalamus
Anxiety
Binding Sites

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Hayashi, Tetsuo ; Yamada, Kiyofumi ; Hasegawa, Takaaki ; Nabeshima, Toshitaka ; Ishihara, Seiichi ; Kameyama, Tsutomu ; Morimasa, Tadaomi ; Kaneyuki, Takao ; Shohmori, Toshikiyo. / Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2) : Changes in GABAA and Benzodiazepine Receptor Binding. In: The Japanese Journal of Pharmacology. 1994 ; Vol. 66, No. 1. pp. 53-58.
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abstract = "We investigated the effects of the long-term administration of Kamikihito (KKT) on the specific binding of [3H]muscimol and [3H]fhmitrazepam in the brains of young and aged rats using in vitro quantitative autoradiography. Specific [3H]muscimol binding in aged rats was decreased in all brain regions examined compared with that in young rats, whereas [3H]flunitrazepam binding did not change in any of the brain regions. Scatchard analysis revealed that the maximal number of [3H]muscimol binding sites in the cortex and thalamus was significantly decreased in aged rats compared with young rats, while its affinity remained unchanged. Long-term administration of KKT in young rats had no effect on either [3H]muscimol or [3H]-flunitrazepam binding. In contrast, the same treatment in aged rats produced a significant increase in [3H]-flunitrazepam binding to the cortex, caudate/putamen and accumbens, and it tended to decrease the [3H]-muscimol binding. These results suggest that the selective reduction of specific [3H]muscimol binding in the brain may be responsible, at least in part, for anxiety-related behavior in aged rats. Furthermore, it appears that the significant increase in specific [3H]flunitrazepam binding produced in the brains of aged rats by the long-term administration of KKT may be responsible for the anxiolytic effects of this agent.",
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Effects of Kamikihito, a Traditional Chinese Medicine, on Neurotransmitter Receptor Binding in the Aged Rat Brain Determined by In Vitro Autoradiography (2) : Changes in GABAA and Benzodiazepine Receptor Binding. / Hayashi, Tetsuo; Yamada, Kiyofumi; Hasegawa, Takaaki; Nabeshima, Toshitaka; Ishihara, Seiichi; Kameyama, Tsutomu; Morimasa, Tadaomi; Kaneyuki, Takao; Shohmori, Toshikiyo.

In: The Japanese Journal of Pharmacology, Vol. 66, No. 1, 01.01.1994, p. 53-58.

Research output: Contribution to journalArticle

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AU - Hayashi, Tetsuo

AU - Yamada, Kiyofumi

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AU - Ishihara, Seiichi

AU - Kameyama, Tsutomu

AU - Morimasa, Tadaomi

AU - Kaneyuki, Takao

AU - Shohmori, Toshikiyo

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