Effects of plasma glycosyltransferase on the ABO(H) blood group antigens of human von Willebrand factor

Taiki Kano, Kazunao Kondo, Jiharu Hamako, Fumio Matsushita, Kazuya Sakai, Taei Matsui

Research output: Contribution to journalArticle

Abstract

Von Willebrand factor (VWF) is one of the plasma protein carrying ABO(H) blood group antigens, but the combining process of these antigens is not clear. In the present study, we examined whether plasma glycosyltransferase affects the blood group antigens on VWF. VWF expressing H-antigen (H-VWF) from blood group O and bovine serum albumin conjugated with H-antigen (H-BSA) were incubated with recombinant α1-3-N-acetylgalactosaminyltransferase (rA-transferase) and A-plasma with or without an additional UDP-GalNAc. Transformed antigens were detected by western blotting and ELISA, using an anti-A antibody. Both H-VWF and H-BSA acquired the A-antigen after incubation with rA-transferase and UDP-GalNAc. Incubation with A-plasma very weakly converted the H-antigen on BSA and VWF to A-antigen only in the presence of supplemented UDP-GalNAc. This conversion was enhanced on desialylation of H-VWF. These results indicate that sugar chains of plasma VWF can be modified by the external glycosyltransferase, but that plasma glycosyltransferase has no effect on the blood group antigens of VWF due to its low activity and the lack of donor sugars. Further, sialic acid residues of VWF may exert a protective effect against post-translational glycosylation. Our results clearly exclude the possibility that blood group antigens of VWF are constructed extracellularly in plasma.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalInternational Journal of Hematology
Volume108
Issue number2
DOIs
Publication statusPublished - 01-08-2018

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ABO Blood-Group System
Glycosyltransferases
von Willebrand Factor
Blood Group Antigens
Uridine Diphosphate
N-Acetylgalactosaminyltransferases
Antigens
Complement Factor H
N-Acetylneuraminic Acid
Bovine Serum Albumin
Glycosylation
Blood Proteins
Anti-Idiotypic Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Kano, Taiki ; Kondo, Kazunao ; Hamako, Jiharu ; Matsushita, Fumio ; Sakai, Kazuya ; Matsui, Taei. / Effects of plasma glycosyltransferase on the ABO(H) blood group antigens of human von Willebrand factor. In: International Journal of Hematology. 2018 ; Vol. 108, No. 2. pp. 139-144.
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Effects of plasma glycosyltransferase on the ABO(H) blood group antigens of human von Willebrand factor. / Kano, Taiki; Kondo, Kazunao; Hamako, Jiharu; Matsushita, Fumio; Sakai, Kazuya; Matsui, Taei.

In: International Journal of Hematology, Vol. 108, No. 2, 01.08.2018, p. 139-144.

Research output: Contribution to journalArticle

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AU - Kondo, Kazunao

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