Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats

Yuji Morine, Runihiro Tsuchida, Hiromu Sugino, Kazuto Takamura, Seiki Tashiro, Mitsuo Shimada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aims: We performed a long-term assessment of liver regeneration with or without PVL after massive hepatectomy in similar sized remnant livers to evaluate effects of regenerating livers preactivated by PVL following massive hepatectomy. Methodology: Rats were randomly divided into two groups, PVL-88%Hx and sham 88%Hx. As the initial operation, PVL or sham operation was performed by ligation of the portal vein of the left and median lobes or a similar manipulation without ligation, respectively. Four days after PVL, the volume of the posterior caudate lobe (5%) increased approximately two-fold (12%) and was the same size as the whole caudate lobe (12%) in the sham animals. Subsequently, 88%Hx was performed in the two groups. Results: Survival rates were not significantly different between the two groups. Relative liver weight in PVL-88%Hx group was significantly higher up to 24hr, but after 48hr no significant difference was evident between the two groups. PCNA LI in sham-89%Hx group was significantly higher than that in PVL-88%Hx group after 48hr. The mRNA expression levels of activin A and ActRIIA were significantly higher in PVL-88%Hx group than in sham-88% group at 72hr. Conclusions: The regenerating liver preactivated by PVL is restricted late-phase liver regeneration after massive hepatectomy.

Original languageEnglish
Pages (from-to)1216-1221
Number of pages6
JournalHepato-gastroenterology
Volume54
Issue number76
Publication statusPublished - 01-06-2007

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Liver Regeneration
Hepatectomy
Portal Vein
Ligation
Liver
Proliferating Cell Nuclear Antigen
Weights and Measures
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Morine, Yuji ; Tsuchida, Runihiro ; Sugino, Hiromu ; Takamura, Kazuto ; Tashiro, Seiki ; Shimada, Mitsuo. / Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats. In: Hepato-gastroenterology. 2007 ; Vol. 54, No. 76. pp. 1216-1221.
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abstract = "Background/Aims: We performed a long-term assessment of liver regeneration with or without PVL after massive hepatectomy in similar sized remnant livers to evaluate effects of regenerating livers preactivated by PVL following massive hepatectomy. Methodology: Rats were randomly divided into two groups, PVL-88{\%}Hx and sham 88{\%}Hx. As the initial operation, PVL or sham operation was performed by ligation of the portal vein of the left and median lobes or a similar manipulation without ligation, respectively. Four days after PVL, the volume of the posterior caudate lobe (5{\%}) increased approximately two-fold (12{\%}) and was the same size as the whole caudate lobe (12{\%}) in the sham animals. Subsequently, 88{\%}Hx was performed in the two groups. Results: Survival rates were not significantly different between the two groups. Relative liver weight in PVL-88{\%}Hx group was significantly higher up to 24hr, but after 48hr no significant difference was evident between the two groups. PCNA LI in sham-89{\%}Hx group was significantly higher than that in PVL-88{\%}Hx group after 48hr. The mRNA expression levels of activin A and ActRIIA were significantly higher in PVL-88{\%}Hx group than in sham-88{\%} group at 72hr. Conclusions: The regenerating liver preactivated by PVL is restricted late-phase liver regeneration after massive hepatectomy.",
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Morine, Y, Tsuchida, R, Sugino, H, Takamura, K, Tashiro, S & Shimada, M 2007, 'Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats', Hepato-gastroenterology, vol. 54, no. 76, pp. 1216-1221.

Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats. / Morine, Yuji; Tsuchida, Runihiro; Sugino, Hiromu; Takamura, Kazuto; Tashiro, Seiki; Shimada, Mitsuo.

In: Hepato-gastroenterology, Vol. 54, No. 76, 01.06.2007, p. 1216-1221.

Research output: Contribution to journalArticle

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T1 - Effects of preactivation by portal vein ligation on liver regeneration following massive hepatectomy in rats

AU - Morine, Yuji

AU - Tsuchida, Runihiro

AU - Sugino, Hiromu

AU - Takamura, Kazuto

AU - Tashiro, Seiki

AU - Shimada, Mitsuo

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Y1 - 2007/6/1

N2 - Background/Aims: We performed a long-term assessment of liver regeneration with or without PVL after massive hepatectomy in similar sized remnant livers to evaluate effects of regenerating livers preactivated by PVL following massive hepatectomy. Methodology: Rats were randomly divided into two groups, PVL-88%Hx and sham 88%Hx. As the initial operation, PVL or sham operation was performed by ligation of the portal vein of the left and median lobes or a similar manipulation without ligation, respectively. Four days after PVL, the volume of the posterior caudate lobe (5%) increased approximately two-fold (12%) and was the same size as the whole caudate lobe (12%) in the sham animals. Subsequently, 88%Hx was performed in the two groups. Results: Survival rates were not significantly different between the two groups. Relative liver weight in PVL-88%Hx group was significantly higher up to 24hr, but after 48hr no significant difference was evident between the two groups. PCNA LI in sham-89%Hx group was significantly higher than that in PVL-88%Hx group after 48hr. The mRNA expression levels of activin A and ActRIIA were significantly higher in PVL-88%Hx group than in sham-88% group at 72hr. Conclusions: The regenerating liver preactivated by PVL is restricted late-phase liver regeneration after massive hepatectomy.

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