Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells

Shogo Asano, Atsushi Suzuki, Sahoko Sekiguchi, Keiko Nishiwaki-Yasuda, Megumi Shibata, Mitsuyasu Itoh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Inorganic phosphate (Pi) transport probably represents an important function of bone-forming cells in relation to extracellular matrix mineralization. In the present study, we investigated the effect of prostaglandin D2 (PGD2) on Pi transport activity and its intracellular signaling mechanism in MC3T3-E1 osteoblast-like cells. PGD2 stimulated Na-dependent Pi uptake time- and dose-dependently in MC3T3-E1 cells during their proliferative phase. A protein kinase C (PKC) inhibitor calphostin C partially suppressed the stimulatory effect of PGD2 on Pi uptake. The selective inhibitors of mitogen-activated protein (MAP) kinase pathways such as ERK, p38 and Jun kinases suppressed PGD2-induced Pi uptake. The inhibitors of phosphatidylinositol (PI) 3-kinase and S6 kinase reduced this effect of PGD2, while Akt kinase inhibitor did not. These results suggest that PGD2 stimulates Na-dependent Pi transport activity in the phase of proliferation of osteoblasts. The mechanisms responsible for this effect are activation of PKC, MAP kinases, PI 3-kinase and S6 kinase.

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume81
Issue number4
DOIs
Publication statusPublished - 01-10-2009

Fingerprint

Prostaglandin D2
Osteoblasts
Phosphates
Phosphatidylinositol 3-Kinase
Ribosomal Protein S6 Kinases
Mitogen-Activated Protein Kinases
Protein Kinase C
Phosphotransferases
Protein C Inhibitor
Protein Kinase Inhibitors
Extracellular Matrix
Bone
Chemical activation
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

Cite this

Asano, Shogo ; Suzuki, Atsushi ; Sekiguchi, Sahoko ; Nishiwaki-Yasuda, Keiko ; Shibata, Megumi ; Itoh, Mitsuyasu. / Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2009 ; Vol. 81, No. 4. pp. 247-251.
@article{868c3d7a9ffa452980cc30082e73b65f,
title = "Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells",
abstract = "Inorganic phosphate (Pi) transport probably represents an important function of bone-forming cells in relation to extracellular matrix mineralization. In the present study, we investigated the effect of prostaglandin D2 (PGD2) on Pi transport activity and its intracellular signaling mechanism in MC3T3-E1 osteoblast-like cells. PGD2 stimulated Na-dependent Pi uptake time- and dose-dependently in MC3T3-E1 cells during their proliferative phase. A protein kinase C (PKC) inhibitor calphostin C partially suppressed the stimulatory effect of PGD2 on Pi uptake. The selective inhibitors of mitogen-activated protein (MAP) kinase pathways such as ERK, p38 and Jun kinases suppressed PGD2-induced Pi uptake. The inhibitors of phosphatidylinositol (PI) 3-kinase and S6 kinase reduced this effect of PGD2, while Akt kinase inhibitor did not. These results suggest that PGD2 stimulates Na-dependent Pi transport activity in the phase of proliferation of osteoblasts. The mechanisms responsible for this effect are activation of PKC, MAP kinases, PI 3-kinase and S6 kinase.",
author = "Shogo Asano and Atsushi Suzuki and Sahoko Sekiguchi and Keiko Nishiwaki-Yasuda and Megumi Shibata and Mitsuyasu Itoh",
year = "2009",
month = "10",
day = "1",
doi = "10.1016/j.plefa.2009.06.007",
language = "English",
volume = "81",
pages = "247--251",
journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "4",

}

Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells. / Asano, Shogo; Suzuki, Atsushi; Sekiguchi, Sahoko; Nishiwaki-Yasuda, Keiko; Shibata, Megumi; Itoh, Mitsuyasu.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 81, No. 4, 01.10.2009, p. 247-251.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of prostaglandin D2 on Na-dependent phosphate transport activity and its intracellular signaling mechanism in osteoblast-like cells

AU - Asano, Shogo

AU - Suzuki, Atsushi

AU - Sekiguchi, Sahoko

AU - Nishiwaki-Yasuda, Keiko

AU - Shibata, Megumi

AU - Itoh, Mitsuyasu

PY - 2009/10/1

Y1 - 2009/10/1

N2 - Inorganic phosphate (Pi) transport probably represents an important function of bone-forming cells in relation to extracellular matrix mineralization. In the present study, we investigated the effect of prostaglandin D2 (PGD2) on Pi transport activity and its intracellular signaling mechanism in MC3T3-E1 osteoblast-like cells. PGD2 stimulated Na-dependent Pi uptake time- and dose-dependently in MC3T3-E1 cells during their proliferative phase. A protein kinase C (PKC) inhibitor calphostin C partially suppressed the stimulatory effect of PGD2 on Pi uptake. The selective inhibitors of mitogen-activated protein (MAP) kinase pathways such as ERK, p38 and Jun kinases suppressed PGD2-induced Pi uptake. The inhibitors of phosphatidylinositol (PI) 3-kinase and S6 kinase reduced this effect of PGD2, while Akt kinase inhibitor did not. These results suggest that PGD2 stimulates Na-dependent Pi transport activity in the phase of proliferation of osteoblasts. The mechanisms responsible for this effect are activation of PKC, MAP kinases, PI 3-kinase and S6 kinase.

AB - Inorganic phosphate (Pi) transport probably represents an important function of bone-forming cells in relation to extracellular matrix mineralization. In the present study, we investigated the effect of prostaglandin D2 (PGD2) on Pi transport activity and its intracellular signaling mechanism in MC3T3-E1 osteoblast-like cells. PGD2 stimulated Na-dependent Pi uptake time- and dose-dependently in MC3T3-E1 cells during their proliferative phase. A protein kinase C (PKC) inhibitor calphostin C partially suppressed the stimulatory effect of PGD2 on Pi uptake. The selective inhibitors of mitogen-activated protein (MAP) kinase pathways such as ERK, p38 and Jun kinases suppressed PGD2-induced Pi uptake. The inhibitors of phosphatidylinositol (PI) 3-kinase and S6 kinase reduced this effect of PGD2, while Akt kinase inhibitor did not. These results suggest that PGD2 stimulates Na-dependent Pi transport activity in the phase of proliferation of osteoblasts. The mechanisms responsible for this effect are activation of PKC, MAP kinases, PI 3-kinase and S6 kinase.

UR - http://www.scopus.com/inward/record.url?scp=70349487497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349487497&partnerID=8YFLogxK

U2 - 10.1016/j.plefa.2009.06.007

DO - 10.1016/j.plefa.2009.06.007

M3 - Article

VL - 81

SP - 247

EP - 251

JO - Prostaglandins Leukotrienes and Essential Fatty Acids

JF - Prostaglandins Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 4

ER -