Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages

Dayong Wang; Yukihiro Noda; Yuan Zhou; Atsumi Nitta; Toshitaka Nabeshima; Qinghai Yu

doi:10.1016/j.intimp.2004.05.008

Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages

Dayong Wang, Yukihiro Noda, Yuan Zhou, Atsumi Nitta, Toshitaka Nabeshima, Qinghai Yu

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

The purpose of this research is to investigate the effects of sodium houttuyfonate on the phosphorylation of CaMK II, CREB and ERK 1/2, and the expression of c-Fos. Macrophages were cultured in vitro with or without sodium houttuyfonate in the culture medium. After cell culture, macrophages were lysed and the lysate of the macrophages was collected for analysis. Western-blotting method was adopted to investigate the phosphorylation or the expression of these signal elements. It was found in this research that the phosphorylation levels of CaMK II and CREB and the expression of c-Fos protein in macrophages were increased by sodium houttuyfonate treatment; however, the phosphorylation level of ERK 1/2 was not affected by the treatment.

Original language	English
Pages (from-to)	1083-1088
Number of pages	6
Journal	International Immunopharmacology
Volume	4
Issue number	8
DOIs	https://doi.org/10.1016/j.intimp.2004.05.008
Publication status	Published - 08-2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Pharmacology

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10.1016/j.intimp.2004.05.008

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title = "Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages",

abstract = "The purpose of this research is to investigate the effects of sodium houttuyfonate on the phosphorylation of CaMK II, CREB and ERK 1/2, and the expression of c-Fos. Macrophages were cultured in vitro with or without sodium houttuyfonate in the culture medium. After cell culture, macrophages were lysed and the lysate of the macrophages was collected for analysis. Western-blotting method was adopted to investigate the phosphorylation or the expression of these signal elements. It was found in this research that the phosphorylation levels of CaMK II and CREB and the expression of c-Fos protein in macrophages were increased by sodium houttuyfonate treatment; however, the phosphorylation level of ERK 1/2 was not affected by the treatment.",

author = "Dayong Wang and Yukihiro Noda and Yuan Zhou and Atsumi Nitta and Toshitaka Nabeshima and Qinghai Yu",

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T1 - Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages

AU - Wang, Dayong

AU - Noda, Yukihiro

AU - Zhou, Yuan

AU - Nitta, Atsumi

AU - Nabeshima, Toshitaka

AU - Yu, Qinghai

PY - 2004/8

Y1 - 2004/8

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AB - The purpose of this research is to investigate the effects of sodium houttuyfonate on the phosphorylation of CaMK II, CREB and ERK 1/2, and the expression of c-Fos. Macrophages were cultured in vitro with or without sodium houttuyfonate in the culture medium. After cell culture, macrophages were lysed and the lysate of the macrophages was collected for analysis. Western-blotting method was adopted to investigate the phosphorylation or the expression of these signal elements. It was found in this research that the phosphorylation levels of CaMK II and CREB and the expression of c-Fos protein in macrophages were increased by sodium houttuyfonate treatment; however, the phosphorylation level of ERK 1/2 was not affected by the treatment.

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Effects of sodium houttuyfonate on phosphorylation of CaMK II, CREB and ERK 1/2 and expression of c-Fos in macrophages

Abstract

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