Effects of sucralfate, cimetidine and rabeprazole on mucosal hydroxyproline content in healing of ethanol-HCL-induced gastric lesions

Tomiyasu Arisawa, Tomoyuki Shibata, Yoshio Kamiya, Mitsuo Nagasaka, Masakatsu Nakamura, Hiroshi Fujita, Shin Hasegawa, Masao Harata, Masahiko Nakamura, Tamaki Mizuno, Tomomitsu Tahara, Yoshiji Ohta, Hiroshi Nakano

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Abstract

1. No general consensus has been reached on the treatment of acute gastric lesions. The aims of the present study were to clarify the effects of sucralfate, cimetidine and rabeprazole monotherapies and combination therapies on acute gastric lesions from the viewpoint of connective tissue regeneration. 2. Gastric lesions were experimentally created by the oral administration of 50% ethanol-0.15 mol/L HCl to rats. After 30 min, the anti-ulcer agents sucralfate (100 mg/kg), cimetidine (20 mg/kg) and rabeprazole (2 mg/kg) were administered separately or in combination and the stomach was excised at different times to measure the level of hydroxyproline in the gastric mucosa and determine lesion index. Immunostaining against prolylhydroxylase was performed on some specimens. 3. In the control group, lesion index decreased linearly from 30 min after ethanol-HCl administration and the level of mucosal hydroxyproline peaked between 2 and 4 h later. Although sucralfate significantly promoted lesion healing, it had no effect on mucosal hydroxyproline level. Cimetidine suppressed increases in mucosal hydroxyproline and prolonged lesion healing, but these findings were reversed by combining cimetidine and sucralfate. Rabeprazole had no significant effect on lesion healing, but promoted lesion healing in combination with sucralfate. Immunohistochemical analysis showed that prolylhydroxylase was expressed in spindle cells that lined the glandular cells in a boundary area between normal and injured tissues. 4. Under conditions in which the effects of intragastric pH are minimal, sucralfate is superior to antisecretory agents in promoting the healing of acute gastric lesions.

Original languageEnglish
Pages (from-to)628-632
Number of pages5
JournalClinical and Experimental Pharmacology and Physiology
Volume33
Issue number7
DOIs
Publication statusPublished - 01-07-2006

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Arisawa, T., Shibata, T., Kamiya, Y., Nagasaka, M., Nakamura, M., Fujita, H., Hasegawa, S., Harata, M., Nakamura, M., Mizuno, T., Tahara, T., Ohta, Y., & Nakano, H. (2006). Effects of sucralfate, cimetidine and rabeprazole on mucosal hydroxyproline content in healing of ethanol-HCL-induced gastric lesions. Clinical and Experimental Pharmacology and Physiology, 33(7), 628-632. https://doi.org/10.1111/j.1440-1681.2006.04418.x