TY - JOUR
T1 - Efficacy and safety of human adipose tissue-derived mesenchymal stem cells for supporting hematopoiesis
AU - Nishiwaki, Satoshi
AU - Nakayama, Takayuki
AU - Saito, Shigeki
AU - Mizuno, Hiroki
AU - Ozaki, Takenori
AU - Takahashi, Yoshiyuki
AU - Maruyama, Shoichi
AU - Nishida, Tetsuya
AU - Murata, Makoto
AU - Kojima, Seiji
AU - Naoe, Tomoki
N1 - Funding Information:
Acknowledgments The authors would like to thank Ms. Tomoko Kawake and Ms. Chika Wakamatsu for their technical assistance. This work was Grant Funding: Supported in part by the Japan Leukaemia Research Fund Grant to S.N. and, in part, by a Japanese Grant-in-Aid for Scientific Research [(C) 20591118] to T.N.
PY - 2012/9
Y1 - 2012/9
N2 - We have demonstrated that adipose tissuederived mesenchymal stem cells (ADSCs) from mice are capable of reconstituting the hematopoietic microenvironment, and facilitate hematopoiesis more effectively than bone marrow-derived mesenchymal stem cells (BMSCs) in mouse. The ready accessibility of fat tissue rich in MSCs and the higher hematopoiesis-supporting capacities of ADSCs suggest that ADSCs might represent a new therapeutic modality for the regeneration of impaired hematopoiesis. As a further step towards their use in clinical practice, we established human BMSCs and ADSCs from healthy volunteers of similar age, and compared their proliferation capacities, hematopoiesis-supporting properties, and safety. In vitro cell proliferation studies revealed that ADSCs have a higher population doubling number than BMSCs. In vitro co-culture assays showed that ADSCs not only support human CD34 + peripheral blood stem cells (PBSCs), but also yield significantly more non-adherent hematic cells than BMSCs. In vitro progenitor assays revealed that ADSCs promote a higher frequency of early progenitors than do BMSCs. Interestingly, BM cellularity in irradiated mice that had received ADSCs tended to be higher than that of mice treated with BMSCs. When MSCs were injected into the BM cavity of tibiae, we observed no evidence of MSC-induced toxicity either during or after treatment. In addition, no microscopic abnormalities were observed in the bone marrow and major organs.
AB - We have demonstrated that adipose tissuederived mesenchymal stem cells (ADSCs) from mice are capable of reconstituting the hematopoietic microenvironment, and facilitate hematopoiesis more effectively than bone marrow-derived mesenchymal stem cells (BMSCs) in mouse. The ready accessibility of fat tissue rich in MSCs and the higher hematopoiesis-supporting capacities of ADSCs suggest that ADSCs might represent a new therapeutic modality for the regeneration of impaired hematopoiesis. As a further step towards their use in clinical practice, we established human BMSCs and ADSCs from healthy volunteers of similar age, and compared their proliferation capacities, hematopoiesis-supporting properties, and safety. In vitro cell proliferation studies revealed that ADSCs have a higher population doubling number than BMSCs. In vitro co-culture assays showed that ADSCs not only support human CD34 + peripheral blood stem cells (PBSCs), but also yield significantly more non-adherent hematic cells than BMSCs. In vitro progenitor assays revealed that ADSCs promote a higher frequency of early progenitors than do BMSCs. Interestingly, BM cellularity in irradiated mice that had received ADSCs tended to be higher than that of mice treated with BMSCs. When MSCs were injected into the BM cavity of tibiae, we observed no evidence of MSC-induced toxicity either during or after treatment. In addition, no microscopic abnormalities were observed in the bone marrow and major organs.
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U2 - 10.1007/s12185-012-1140-8
DO - 10.1007/s12185-012-1140-8
M3 - Article
C2 - 22782260
AN - SCOPUS:84867009276
SN - 0925-5710
VL - 96
SP - 295
EP - 300
JO - International Journal of Hematology
JF - International Journal of Hematology
IS - 3
ER -