Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells

Eisei Kondo, Max S. Topp, Hans Peter Kiem, Yuichi Obata, Yasuo Morishima, Kiyotaka Kuzushima, Mitsune Tanimoto, Mine Harada, Toshitada Takahashi, Yoshiki Akatsuka

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 mi peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.

Original languageEnglish
Pages (from-to)2164-2171
Number of pages8
JournalJournal of Immunology
Volume169
Issue number4
DOIs
Publication statusPublished - 15-08-2002

Fingerprint

B-Lymphocytes
T-Lymphocytes
Antigens
Adoptive Immunotherapy
Enzyme-Linked Immunospot Assay
CD40 Ligand
Interleukin-4
Complementary DNA
Alleles
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Kondo, E., Topp, M. S., Kiem, H. P., Obata, Y., Morishima, Y., Kuzushima, K., ... Akatsuka, Y. (2002). Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells. Journal of Immunology, 169(4), 2164-2171. https://doi.org/10.4049/jimmunol.169.4.2164
Kondo, Eisei ; Topp, Max S. ; Kiem, Hans Peter ; Obata, Yuichi ; Morishima, Yasuo ; Kuzushima, Kiyotaka ; Tanimoto, Mitsune ; Harada, Mine ; Takahashi, Toshitada ; Akatsuka, Yoshiki. / Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells. In: Journal of Immunology. 2002 ; Vol. 169, No. 4. pp. 2164-2171.
@article{80f60b745fbf484e8aa2d646856c4faf,
title = "Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells",
abstract = "The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 mi peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.",
author = "Eisei Kondo and Topp, {Max S.} and Kiem, {Hans Peter} and Yuichi Obata and Yasuo Morishima and Kiyotaka Kuzushima and Mitsune Tanimoto and Mine Harada and Toshitada Takahashi and Yoshiki Akatsuka",
year = "2002",
month = "8",
day = "15",
doi = "10.4049/jimmunol.169.4.2164",
language = "English",
volume = "169",
pages = "2164--2171",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

Kondo, E, Topp, MS, Kiem, HP, Obata, Y, Morishima, Y, Kuzushima, K, Tanimoto, M, Harada, M, Takahashi, T & Akatsuka, Y 2002, 'Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells', Journal of Immunology, vol. 169, no. 4, pp. 2164-2171. https://doi.org/10.4049/jimmunol.169.4.2164

Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells. / Kondo, Eisei; Topp, Max S.; Kiem, Hans Peter; Obata, Yuichi; Morishima, Yasuo; Kuzushima, Kiyotaka; Tanimoto, Mitsune; Harada, Mine; Takahashi, Toshitada; Akatsuka, Yoshiki.

In: Journal of Immunology, Vol. 169, No. 4, 15.08.2002, p. 2164-2171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Efficient generation of antigen-specific cytotoxic T cells using retrovirally transduced CD40-activated B cells

AU - Kondo, Eisei

AU - Topp, Max S.

AU - Kiem, Hans Peter

AU - Obata, Yuichi

AU - Morishima, Yasuo

AU - Kuzushima, Kiyotaka

AU - Tanimoto, Mitsune

AU - Harada, Mine

AU - Takahashi, Toshitada

AU - Akatsuka, Yoshiki

PY - 2002/8/15

Y1 - 2002/8/15

N2 - The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 mi peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.

AB - The development of rapid, efficient, and safe methods for generating Ag-specific T cells is necessary for the clinical application of adoptive immunotherapy. We show that B cells stimulated with CD40 ligand and IL-4 (CD40-B cells) can be efficiently transduced with retroviral vectors encoding a model Ag, CMV tegument protein pp65 gene, and maintain high levels of costimulatory molecules after gene transfer. CTL lines specific for pp65 were readily generated in all four healthy CMV-seropositive donors by stimulating autologous CD8+ T cells with these transduced CD40-B cells, both of which were derived from 10 mi peripheral blood. ELISPOT assays revealed that the CTL lines used multiple HLA alleles as restricting elements. Thus, CD40-B cells transduced retrovirally with Ag-encoding cDNA can be potent APC and facilitate to generate Ag-specific CTL in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0037103241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037103241&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.169.4.2164

DO - 10.4049/jimmunol.169.4.2164

M3 - Article

VL - 169

SP - 2164

EP - 2171

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -