Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata, Hiroki Isomura, Yoriko Yamashita, Shigenori Toyama, Yoshitaka Sato, Sanae Nakayama, Ayumi Kudoh, Satoko Iwahori, Teru Kanda, Tatsuya Tsurumi

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Abstract

The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalVirology
Volume389
Issue number1-2
DOIs
Publication statusPublished - 02-06-2009

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All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Murata, T., Isomura, H., Yamashita, Y., Toyama, S., Sato, Y., Nakayama, S., Kudoh, A., Iwahori, S., Kanda, T., & Tsurumi, T. (2009). Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. Virology, 389(1-2), 75-81. https://doi.org/10.1016/j.virol.2009.04.007