Abstract
The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
| Original language | English |
|---|---|
| Pages (from-to) | 75-81 |
| Number of pages | 7 |
| Journal | Virology |
| Volume | 389 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 02-06-2009 |
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All Science Journal Classification (ASJC) codes
- Virology
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Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. / Murata, Takayuki; Isomura, Hiroki; Yamashita, Yoriko; Toyama, Shigenori; Sato, Yoshitaka; Nakayama, Sanae; Kudoh, Ayumi; Iwahori, Satoko; Kanda, Teru; Tsurumi, Tatsuya.
In: Virology, Vol. 389, No. 1-2, 02.06.2009, p. 75-81.Research output: Contribution to journal › Article
TY - JOUR
T1 - Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase
AU - Murata, Takayuki
AU - Isomura, Hiroki
AU - Yamashita, Yoriko
AU - Toyama, Shigenori
AU - Sato, Yoshitaka
AU - Nakayama, Sanae
AU - Kudoh, Ayumi
AU - Iwahori, Satoko
AU - Kanda, Teru
AU - Tsurumi, Tatsuya
PY - 2009/6/2
Y1 - 2009/6/2
N2 - The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
AB - The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
UR - http://www.scopus.com/inward/record.url?scp=67349265475&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349265475&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2009.04.007
DO - 10.1016/j.virol.2009.04.007
M3 - Article
C2 - 19427010
AN - SCOPUS:67349265475
VL - 389
SP - 75
EP - 81
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1-2
ER -