TY - JOUR
T1 - Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase
AU - Murata, Takayuki
AU - Isomura, Hiroki
AU - Yamashita, Yoriko
AU - Toyama, Shigenori
AU - Sato, Yoshitaka
AU - Nakayama, Sanae
AU - Kudoh, Ayumi
AU - Iwahori, Satoko
AU - Kanda, Teru
AU - Tsurumi, Tatsuya
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009
Y1 - 2009
N2 - The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
AB - The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.
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U2 - 10.1016/j.virol.2009.04.007
DO - 10.1016/j.virol.2009.04.007
M3 - Article
C2 - 19427010
AN - SCOPUS:67349265475
VL - 389
SP - 75
EP - 81
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1-2
ER -