Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata, Hiroki Isomura, Yoriko Yamashita, Shigenori Toyama, Yoshitaka Sato, Sanae Nakayama, Ayumi Kudoh, Satoko Iwahori, Teru Kanda, Tatsuya Tsurumi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalVirology
Volume389
Issue number1-2
DOIs
Publication statusPublished - 02-06-2009

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Human Herpesvirus 4
Protein Kinases
Viruses
Phosphotransferases
Viral DNA
Viral Load
DNA Replication
Proteins
Genome
Genes

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Murata, Takayuki ; Isomura, Hiroki ; Yamashita, Yoriko ; Toyama, Shigenori ; Sato, Yoshitaka ; Nakayama, Sanae ; Kudoh, Ayumi ; Iwahori, Satoko ; Kanda, Teru ; Tsurumi, Tatsuya. / Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. In: Virology. 2009 ; Vol. 389, No. 1-2. pp. 75-81.
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Murata, T, Isomura, H, Yamashita, Y, Toyama, S, Sato, Y, Nakayama, S, Kudoh, A, Iwahori, S, Kanda, T & Tsurumi, T 2009, 'Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase', Virology, vol. 389, no. 1-2, pp. 75-81. https://doi.org/10.1016/j.virol.2009.04.007

Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. / Murata, Takayuki; Isomura, Hiroki; Yamashita, Yoriko; Toyama, Shigenori; Sato, Yoshitaka; Nakayama, Sanae; Kudoh, Ayumi; Iwahori, Satoko; Kanda, Teru; Tsurumi, Tatsuya.

In: Virology, Vol. 389, No. 1-2, 02.06.2009, p. 75-81.

Research output: Contribution to journalArticle

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AU - Sato, Yoshitaka

AU - Nakayama, Sanae

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AU - Iwahori, Satoko

AU - Kanda, Teru

AU - Tsurumi, Tatsuya

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