Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata; Hiroki Isomura; Yoriko Yamashita; Shigenori Toyama; Yoshitaka Sato; Sanae Nakayama; Ayumi Kudoh; Satoko Iwahori; Teru Kanda; Tatsuya Tsurumi

doi:10.1016/j.virol.2009.04.007

Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata, Hiroki Isomura, Yoriko Yamashita, Shigenori Toyama, Yoshitaka Sato, Sanae Nakayama, Ayumi Kudoh, Satoko Iwahori, Teru Kanda, Tatsuya Tsurumi

Department of Virology and Parasitology

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Virology
Volume	389
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virol.2009.04.007
Publication status	Published - 02-06-2009

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All Science Journal Classification (ASJC) codes

Virology

Cite this

Murata, T., Isomura, H., Yamashita, Y., Toyama, S., Sato, Y., Nakayama, S., Kudoh, A., Iwahori, S., Kanda, T., & Tsurumi, T. (2009). Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. Virology, 389(1-2), 75-81. https://doi.org/10.1016/j.virol.2009.04.007

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Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. / Murata, Takayuki; Isomura, Hiroki; Yamashita, Yoriko; Toyama, Shigenori; Sato, Yoshitaka; Nakayama, Sanae; Kudoh, Ayumi; Iwahori, Satoko; Kanda, Teru; Tsurumi, Tatsuya.

In: Virology, Vol. 389, No. 1-2, 02.06.2009, p. 75-81.

Research output: Contribution to journal › Article

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10.1016/j.virol.2009.04.007