Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata; Hiroki Isomura; Yoriko Yamashita; Shigenori Toyama; Yoshitaka Sato; Sanae Nakayama; Ayumi Kudoh; Satoko Iwahori; Teru Kanda; Tatsuya Tsurumi

doi:10.1016/j.virol.2009.04.007

Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

Takayuki Murata, Hiroki Isomura, Yoriko Yamashita, Shigenori Toyama, Yoshitaka Sato, Sanae Nakayama, Ayumi Kudoh, Satoko Iwahori, Teru Kanda, Tatsuya Tsurumi

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.

Original language	English
Pages (from-to)	75-81
Number of pages	7
Journal	Virology
Volume	389
Issue number	1-2
DOIs	https://doi.org/10.1016/j.virol.2009.04.007
Publication status	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

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title = "Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase",

abstract = "The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.",

author = "Takayuki Murata and Hiroki Isomura and Yoriko Yamashita and Shigenori Toyama and Yoshitaka Sato and Sanae Nakayama and Ayumi Kudoh and Satoko Iwahori and Teru Kanda and Tatsuya Tsurumi",

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language = "English",

volume = "389",

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Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase. / Murata, Takayuki; Isomura, Hiroki; Yamashita, Yoriko; Toyama, Shigenori; Sato, Yoshitaka; Nakayama, Sanae; Kudoh, Ayumi; Iwahori, Satoko; Kanda, Teru; Tsurumi, Tatsuya.

In: Virology, Vol. 389, No. 1-2, 2009, p. 75-81.

Research output: Contribution to journal › Article › peer-review

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T1 - Efficient production of infectious viruses requires enzymatic activity of Epstein-Barr virus protein kinase

AU - Murata, Takayuki

AU - Isomura, Hiroki

AU - Yamashita, Yoriko

AU - Toyama, Shigenori

AU - Sato, Yoshitaka

AU - Nakayama, Sanae

AU - Kudoh, Ayumi

AU - Iwahori, Satoko

AU - Kanda, Teru

AU - Tsurumi, Tatsuya

PY - 2009

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AB - The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant were equivalent to those of the wild-type and the revertant, virus production was significantly impaired. Expression of the BGLF4 protein in trans fully complemented the low yield of the mutant virus, while expression of a kinase-dead (K102I) form of the protein failed to restore the virus titer. These results demonstrate that BGLF4 plays a significant role in production of infectious viruses and that the kinase activity is crucial.

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