Efficient purification of a full length and biochemically active p110Rb, the retinoblastoma gene product

Ericka Savoysky, Motoshi Suzuki, Katsuyuki Tamai, Takeshi Sakurai, Tohru Ohuchi, Tetsu Akiyama, Kyohide Kojima, Shonen Yoshida

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rb protein was purified from recombinant baculovirus-infected Sf9 cells to apparent homogeneity by a simple solubilization and by immunoaffinity column chromatography. It was mainly obtained as p110Rb, the underphosphorylated form of the Rb protein family. This p110Rb was shown to form a specific complex in vitro with SV40 Tag and to bind to double or single stranded DNA. It could also affect Tag helicase activity in a biphasic-dose dependent manner, due to these two biochemical functions. This purification procedure provides sufficient amounts of native Rb protein to further elucidate its role as an anti-oncogene protein and a negative regulator of the cell cycle.

Original languageEnglish
Pages (from-to)697-702
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume187
Issue number2
DOIs
Publication statusPublished - 16-09-1992

Fingerprint

Retinoblastoma Genes
Retinoblastoma Protein
Purification
Genes
Sf9 Cells
Column chromatography
Baculoviridae
Single-Stranded DNA
Oncogene Proteins
Tumor Suppressor Genes
Chromatography
Cell Cycle
Cells
DNA

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Savoysky, Ericka ; Suzuki, Motoshi ; Tamai, Katsuyuki ; Sakurai, Takeshi ; Ohuchi, Tohru ; Akiyama, Tetsu ; Kojima, Kyohide ; Yoshida, Shonen. / Efficient purification of a full length and biochemically active p110Rb, the retinoblastoma gene product. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 187, No. 2. pp. 697-702.
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Efficient purification of a full length and biochemically active p110Rb, the retinoblastoma gene product. / Savoysky, Ericka; Suzuki, Motoshi; Tamai, Katsuyuki; Sakurai, Takeshi; Ohuchi, Tohru; Akiyama, Tetsu; Kojima, Kyohide; Yoshida, Shonen.

In: Biochemical and Biophysical Research Communications, Vol. 187, No. 2, 16.09.1992, p. 697-702.

Research output: Contribution to journalArticle

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