TY - JOUR
T1 - Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers
AU - Hara, Toshifumi
AU - Tominaga, Yuuki
AU - Ueda, Koji
AU - Mihara, Keichiro
AU - Yanagihara, Kazuyoshi
AU - Takei, Yoshifumi
N1 - Publisher Copyright:
© 2022
PY - 2022/7
Y1 - 2022/7
N2 - Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, little is known about the relationship between methyltransferase and cancers. Here we aimed to understand the role of methyltransferase in cancer metastasis. We found that elevated methyltransferase-like 9 (METTL9) is closely associated with the acquisition of metastatic activity in human scirrhous gastric cancers. The stable knockdown of METTL9 via an shRNA vector technique in our original metastatic cells from scirrhous gastric cancer patients significantly inhibited migration and invasion. In metastatic cells, METTL9 protein is predominantly localized in mitochondria, and the METTL9 knockdown significantly reduced mitochondrial Complex I activity. METTL9 can be a candidate of molecular targets to inhibit peritoneal dissemination of scirrhous gastric cancers. This report is the first to describe the relationship between METTL9 and cancer metastasis.
AB - Methylation, the most common chemical modification of cellular components such as DNA, RNA, and proteins, impacts biological processes including transcription, RNA processing, and protein dynamics. Although abnormal expression of methyltransferase can lead to various diseases including cancers, little is known about the relationship between methyltransferase and cancers. Here we aimed to understand the role of methyltransferase in cancer metastasis. We found that elevated methyltransferase-like 9 (METTL9) is closely associated with the acquisition of metastatic activity in human scirrhous gastric cancers. The stable knockdown of METTL9 via an shRNA vector technique in our original metastatic cells from scirrhous gastric cancer patients significantly inhibited migration and invasion. In metastatic cells, METTL9 protein is predominantly localized in mitochondria, and the METTL9 knockdown significantly reduced mitochondrial Complex I activity. METTL9 can be a candidate of molecular targets to inhibit peritoneal dissemination of scirrhous gastric cancers. This report is the first to describe the relationship between METTL9 and cancer metastasis.
KW - Methyltransferase-like (METTL) gene
KW - Molecular targeting therapy against cancer metastasis
KW - Peritoneal dissemination
KW - Scirrhous gastric cancer
KW - Short hairpin RNA
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U2 - 10.1016/j.bbrep.2022.101255
DO - 10.1016/j.bbrep.2022.101255
M3 - Article
AN - SCOPUS:85127346701
SN - 2405-5808
VL - 30
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101255
ER -