Elevation of cytoplasmic free calcium concentration by stable thromboxane A2 analogue in human platelets

Yasuhiro Kawahara, Junji Yamanishi, Yutaka Furuta, Kozo Kaibuchi, Yoshimi Takai, Hisashi Fukuzaki

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

9, 11-Epithio-11, 12-methano-thromboxane A2 (STA2), a stable analogue of thromboxane A2, caused a rapid rise in cytoplasmic free Ca2+ concentration ([Ca2+]i) in human platelets as measured with the fluorescent Ca2+ indicator quin2. Concomitantly, this compound induced phosphorylation of myosin light chain which is catalyzed by Ca2+, calmodulin-dependent protein kinase. These reactions were fast enough to trigger serotonin release. 13-Azaprostanoic acid, a receptor level antagonist of thromboxane A2 inhibited STA2-induced elevation of [Ca2+]i, phosphorylation of myosin light chain and serotonin release. These results provide evidence that STA2 interacts with a thromboxane A2 receptor which leads to elevation of [Ca2+]i.

Original languageEnglish
Pages (from-to)663-669
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume117
Issue number3
DOIs
Publication statusPublished - 28-12-1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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