TY - JOUR
T1 - Elevation of cytoplasmic free calcium concentration by stable thromboxane A2 analogue in human platelets
AU - Kawahara, Yasuhiro
AU - Yamanishi, Junji
AU - Furuta, Yutaka
AU - Kaibuchi, Kozo
AU - Takai, Yoshimi
AU - Fukuzaki, Hisashi
N1 - Funding Information:
*/ This investigation was supported in part by research grants from the Scientific Research Fund of the Ministry of Education, Science and Culture (1982) and the Mitsuhisa Cancer Research Foundation (1982). l/ The abbreviations used are: TXA2, thromboxane A2; U-46619, 9, ll-metTianoepoxyp rostaglandin HZ; CTA2, carbocyclic thromboxane A2; [Ca2+]i, cytoplasmic free Ca2+ concentration; 13-APA, 13-azaprostanoic acid; SDS, sodium dodecyl sulfate; PGE1, prostaglandin El.
PY - 1983/12/28
Y1 - 1983/12/28
N2 - 9, 11-Epithio-11, 12-methano-thromboxane A2 (STA2), a stable analogue of thromboxane A2, caused a rapid rise in cytoplasmic free Ca2+ concentration ([Ca2+]i) in human platelets as measured with the fluorescent Ca2+ indicator quin2. Concomitantly, this compound induced phosphorylation of myosin light chain which is catalyzed by Ca2+, calmodulin-dependent protein kinase. These reactions were fast enough to trigger serotonin release. 13-Azaprostanoic acid, a receptor level antagonist of thromboxane A2 inhibited STA2-induced elevation of [Ca2+]i, phosphorylation of myosin light chain and serotonin release. These results provide evidence that STA2 interacts with a thromboxane A2 receptor which leads to elevation of [Ca2+]i.
AB - 9, 11-Epithio-11, 12-methano-thromboxane A2 (STA2), a stable analogue of thromboxane A2, caused a rapid rise in cytoplasmic free Ca2+ concentration ([Ca2+]i) in human platelets as measured with the fluorescent Ca2+ indicator quin2. Concomitantly, this compound induced phosphorylation of myosin light chain which is catalyzed by Ca2+, calmodulin-dependent protein kinase. These reactions were fast enough to trigger serotonin release. 13-Azaprostanoic acid, a receptor level antagonist of thromboxane A2 inhibited STA2-induced elevation of [Ca2+]i, phosphorylation of myosin light chain and serotonin release. These results provide evidence that STA2 interacts with a thromboxane A2 receptor which leads to elevation of [Ca2+]i.
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U2 - 10.1016/0006-291X(83)91648-0
DO - 10.1016/0006-291X(83)91648-0
M3 - Article
C2 - 6230078
AN - SCOPUS:0021090402
SN - 0006-291X
VL - 117
SP - 663
EP - 669
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -