Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells

Yoshitaka Sato, Shingo Ochiai, Takayuki Murata, Teru Kanda, Fumi Goshima, Hiroshi Kimura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) latently infects malignant epithelial cells in approximately 10% of all gastric cancers. Latent membrane protein 1 (LMP1), an oncogenic protein, plays an important role in malignant transformation in EBV-associated nasopharyngeal carcinoma and B-cell lymphoma; however, its expression has not been detected in EBVassociated gastric cancer. To address why LMP1 has not been detected in EBV-positive gastric tumors, we focused on the interactions between LMP1-positive and -negative cells and stably expressed LMP1 in the gastric cancer cell line AGS. We showed that the number of LMP1-positive cells decreased gradually with each cell passage when the cells were co-cultured with LMP1-negative cells. Time-lapse imaging showed that LMP1-positive cells were eliminated from a monolayer of LMP1-negative cells. Furthermore, LMP1-positive cells stimulated the proliferation of surrounding LMP1- negative cells, but not LMP1-positive cells, via exosome-mediated EGFR activation. Our data indicate that LMP1 expression drives cell competition between LMP1-positive and -negative cells, affecting the behavior of the cells within gastric tissue.

Original languageEnglish
Pages (from-to)39345-39355
Number of pages11
JournalOncotarget
Volume8
Issue number24
DOIs
Publication statusPublished - 01-01-2017

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Stomach Neoplasms
Membrane Proteins
Human Herpesvirus 4
Stomach
Time-Lapse Imaging
Exosomes
B-Cell Lymphoma
Epithelial Cells
Cell Proliferation
Cell Line

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Sato, Yoshitaka ; Ochiai, Shingo ; Murata, Takayuki ; Kanda, Teru ; Goshima, Fumi ; Kimura, Hiroshi. / Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells. In: Oncotarget. 2017 ; Vol. 8, No. 24. pp. 39345-39355.
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abstract = "Epstein-Barr virus (EBV) latently infects malignant epithelial cells in approximately 10{\%} of all gastric cancers. Latent membrane protein 1 (LMP1), an oncogenic protein, plays an important role in malignant transformation in EBV-associated nasopharyngeal carcinoma and B-cell lymphoma; however, its expression has not been detected in EBVassociated gastric cancer. To address why LMP1 has not been detected in EBV-positive gastric tumors, we focused on the interactions between LMP1-positive and -negative cells and stably expressed LMP1 in the gastric cancer cell line AGS. We showed that the number of LMP1-positive cells decreased gradually with each cell passage when the cells were co-cultured with LMP1-negative cells. Time-lapse imaging showed that LMP1-positive cells were eliminated from a monolayer of LMP1-negative cells. Furthermore, LMP1-positive cells stimulated the proliferation of surrounding LMP1- negative cells, but not LMP1-positive cells, via exosome-mediated EGFR activation. Our data indicate that LMP1 expression drives cell competition between LMP1-positive and -negative cells, affecting the behavior of the cells within gastric tissue.",
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Sato, Y, Ochiai, S, Murata, T, Kanda, T, Goshima, F & Kimura, H 2017, 'Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells', Oncotarget, vol. 8, no. 24, pp. 39345-39355. https://doi.org/10.18632/oncotarget.16996

Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells. / Sato, Yoshitaka; Ochiai, Shingo; Murata, Takayuki; Kanda, Teru; Goshima, Fumi; Kimura, Hiroshi.

In: Oncotarget, Vol. 8, No. 24, 01.01.2017, p. 39345-39355.

Research output: Contribution to journalArticle

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