Elongation factor-1α is a novel substrate of Rho-associated kinase

Tetsuyuki Izawa, Yuko Fukata, Toshihide Kimura, Akihiro Iwamatsu, Kazuhiro Dohi, Kozo Kaibuchi

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Rho-associated kinase (Rho-kinase), which is activated by the Rho small GTPase, phosphorylates the myosin-binding subunit (MBS) of myosin phosphatase, myosin light chain (MLC), the ERM family proteins, and adducin, thereby regulating the formation of stress fibers, focal adhesions, microvillus formation, and cell motility. Here, to further understand the role of Rho-kinase in the regulation of the numerous cellular processes by Rho, we purified a novel substrate of Rho-kinase having a molecular mass of 48 kDa (p48) from a rat liver cytosol extract. Mass spectral analysis revealed p48 to be elongation factor-1 alpha (EF-1α), which is known as an actin-binding protein besides a cofactor of polypeptide elongation. Rho-kinase directly phosphorylated recombinant EF-1α in vitro. A high-speed cosedimentation assay revealed that phosphorylation of EF-1α by Rho-kinase decreased the binding activity of EF-1α to filamentous actin (F-actin). A low-speed sedimentation assay revealed that phosphorylation of EF-1α by Rho-kinase decreased the F-actin-bundling activity. In addition, EF-1α bound to MBS of myosin phosphatase, suggesting that both Rho-kinase and myosin phosphatase regulate the phosphorylation state of EF-1α downstream of Rho as other substrates of Rho-kinase, i.e., MLC, adducin, and the ERM family. These results suggest that the Rho/Rho-kinase pathway regulates the organization of actin cytoskeleton via the phosphorylation of EF-1α. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)72-78
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume278
Issue number1
DOIs
Publication statusPublished - 11-11-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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