Embryonic β-Catenin Is Required for Priming of the Uterus to Implantation

Youki Takezawa, Maki Iwai, Yukiko Fujiki, Ryo Yokomizo, Harue Kishigami, Mami Miyado, Natsuko Kawano, Mitsutoshi Yamada, Miyuki Shindo, Miki Suzuki, Ban Sato, Daiki Katano, Shintaro Kamijo, Toshio Hamatani, Mamoru Tanaka, Akihiro Umezawa, Woojin Kang, Kenji Miyado

Research output: Contribution to journalArticlepeer-review


Repeated implantation failure is a major cause of infertility among healthy women. Uterine β-catenin (CTNNB1) plays a critical role in implantation. However, the role of embryonic CTNNB1 during implantation remains unclear. We addressed this topic by analyzing mice carrying Ctnnb1-deficient (Ctnnb1Δ/Δ) embryos. Ctnnb1Δ/Δ embryos were produced by intercrossing mice bearing Ctnnb1-deficient eggs and sperms. We found that Ctnnb1Δ/Δ embryos developed to the blastocyst stage; thereafter, they were resorbed, leaving empty decidual capsules. Moreover, leukemia inhibitory factor, a uterine factor essential for implantation, was undetectable in Ctnnb1Δ/Δ blastocysts. Furthermore, CDX2, a transcription factor that determines the fate of trophectoderm cells, was not observed in Ctnnb1Δ/Δ blastocysts. Intrauterine injection with uterine fluids (from control mice) and recombinant mouse leukemia inhibitory factor proteins rescued the uterine response to Ctnnb1Δ/Δ blastocysts. These results suggest that embryonic CTNNB1 is required for the secretion of blastocyst-derived factor(s) that open the implantation window, indicating that the uterine response to implantation can be induced using supplemental materials. Therefore, our results may contribute to the discovery of a similar mechanism in humans, leading to a better understanding of the pathogenesis of repeated implantation failure.

Original languageEnglish
Article number100026
JournalLaboratory Investigation
Issue number3
Publication statusPublished - 03-2023
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine


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