TY - JOUR
T1 - Emergence of non-classical genotype constellations of G9P[8] rotavirus strains in diarrheic children in Sabah, Malaysia
AU - Fong, Siat Yee
AU - Akari, Yuki
AU - Amit, Lia Natasha
AU - John, Jecelyn Leaslie
AU - Chin, Abraham Zefong
AU - Komoto, Satoshi
AU - Ahmed, Kamruddin
N1 - Publisher Copyright:
© 2024
PY - 2024/11
Y1 - 2024/11
N2 - G9P[8] has been the predominant rotavirus A (RVA) genotype in Malaysia since the 2000s. However, the overall genetic makeup and evolution of Malaysian G9P[8] strains are still unknown. Therefore, this study aimed to evaluate and characterize the complete genomes of three G9P[8] RVA strains isolated from diarrheic children under five years old in Sabah. Contrary to the classical Wa-like constellation, these strains contained a DS-1-like genotype. Two strains, namely L202 and L234, were genotype G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1, while one (KN102) was genotype G9-P[8]-I1-R1-C1-M1-A2-N1-T1-E1-H1. Phylogenetic analysis revealed that the NSP4 genes of L202 and L234 strains were closer to that of G9P[8]-E2 strains from Japan, suggesting they might share a common ancestor. The findings from this study provide new insights into the genetic characteristics of circulating G9P[8] strains in Sabah, which are important for rotavirus surveillance and potential vaccine development in the region.
AB - G9P[8] has been the predominant rotavirus A (RVA) genotype in Malaysia since the 2000s. However, the overall genetic makeup and evolution of Malaysian G9P[8] strains are still unknown. Therefore, this study aimed to evaluate and characterize the complete genomes of three G9P[8] RVA strains isolated from diarrheic children under five years old in Sabah. Contrary to the classical Wa-like constellation, these strains contained a DS-1-like genotype. Two strains, namely L202 and L234, were genotype G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1, while one (KN102) was genotype G9-P[8]-I1-R1-C1-M1-A2-N1-T1-E1-H1. Phylogenetic analysis revealed that the NSP4 genes of L202 and L234 strains were closer to that of G9P[8]-E2 strains from Japan, suggesting they might share a common ancestor. The findings from this study provide new insights into the genetic characteristics of circulating G9P[8] strains in Sabah, which are important for rotavirus surveillance and potential vaccine development in the region.
KW - G9P[8]-A2
KW - G9P[8]-E2
KW - Malaysia
KW - Reassortment
KW - Rotavirus
KW - Whole-genome
UR - http://www.scopus.com/inward/record.url?scp=85207346261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85207346261&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2024.105685
DO - 10.1016/j.meegid.2024.105685
M3 - Article
C2 - 39461399
AN - SCOPUS:85207346261
SN - 1567-1348
VL - 125
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
M1 - 105685
ER -