TY - JOUR
T1 - Endometrial regeneration using cell sheet transplantation techniques in rats facilitates successful fertilization and pregnancy
AU - Kuramoto, Goro
AU - Shimizu, Tatsuya
AU - Takagi, Soichi
AU - Ishitani, Ken
AU - Matsui, Hideo
AU - Okano, Teruo
N1 - Publisher Copyright:
© 2018 American Society for Reproductive Medicine
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objective: To regenerate functional endometrium tissue using “cell sheet” techniques as a regenerative medicine approach to address endometrial disorders causing female factor infertility. Design: In vivo experimental study. Setting: Preclinical surgical and biomedical research laboratories. Animal(s): Green fluorescent protein (GFP) transgenic rats [SD-Tg (CAG-EGFP) rats] and nude rats (F344/NJcl-rnu/rnu). Intervention(s): GFP-positive rat uterine-derived cells as cell sheets were transplanted into resected rat uterine endometrial sites. Transplanted cell sheet areas were then analyzed using macroscopic observations and histological analysis including immunohistochemistry. Subsequently, crossbreeding was performed to establish fertility and confirm pregnancy in the rat-regenerated uterus. Main Outcome Measure(s): Morphologic and biochemical markers of regenerated endometrium and establishment of pregnancy in otherwise sterile animals. Result(s): After cell sheet transplantation, regenerated endometrium was confirmed as GFP-positive tissue engraftment both visually and under histological analysis. After crossbreeding, GFP-positive tissue areas and living fetuses were observed in the transplantation group. Conclusion(s): Cell sheet transplantation can regenerate endometrial tissue with histological structure and physiological function supporting pregnancy similar to normal endometrial tissue. Translation of this endometrial cell sheet transplantation method to human patients with endometrial disorders could yield a novel therapy for uterine infertility.
AB - Objective: To regenerate functional endometrium tissue using “cell sheet” techniques as a regenerative medicine approach to address endometrial disorders causing female factor infertility. Design: In vivo experimental study. Setting: Preclinical surgical and biomedical research laboratories. Animal(s): Green fluorescent protein (GFP) transgenic rats [SD-Tg (CAG-EGFP) rats] and nude rats (F344/NJcl-rnu/rnu). Intervention(s): GFP-positive rat uterine-derived cells as cell sheets were transplanted into resected rat uterine endometrial sites. Transplanted cell sheet areas were then analyzed using macroscopic observations and histological analysis including immunohistochemistry. Subsequently, crossbreeding was performed to establish fertility and confirm pregnancy in the rat-regenerated uterus. Main Outcome Measure(s): Morphologic and biochemical markers of regenerated endometrium and establishment of pregnancy in otherwise sterile animals. Result(s): After cell sheet transplantation, regenerated endometrium was confirmed as GFP-positive tissue engraftment both visually and under histological analysis. After crossbreeding, GFP-positive tissue areas and living fetuses were observed in the transplantation group. Conclusion(s): Cell sheet transplantation can regenerate endometrial tissue with histological structure and physiological function supporting pregnancy similar to normal endometrial tissue. Translation of this endometrial cell sheet transplantation method to human patients with endometrial disorders could yield a novel therapy for uterine infertility.
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U2 - 10.1016/j.fertnstert.2018.03.007
DO - 10.1016/j.fertnstert.2018.03.007
M3 - Article
C2 - 29980256
AN - SCOPUS:85049322191
SN - 0015-0282
VL - 110
SP - 172-181.e4
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 1
ER -