Engulfment of spermatozoa by cumulus cells and the role of CD36

X. Z. Yang, K. Kumamoto, A. Hasegawa, S. Komori, K. Koyama

Research output: Contribution to journalArticle

Abstract

The function of cumulus cells after sperm penetration is not well understood. The present study examined the phagocytic action of cumulus cells on sperm after dispersion of cumulus-oocyte complexes. In a co-incubation system of cauda sperm and cumulus cells, the sperm heads were beginning to vanish after 2.5 h and 77% ± 1.34 of sperm heads had disappeared at 30. h. Most of the sperm heads were engulfed by cumulus cells. Immunofluorescent studies showed that cumulus cells were expressing the CD36 molecule, and sperm were exposing phosphatidylserine (PS). Anti-CD36 antibody and annexin V inhibited the engulfment of sperm by cumulus cells by 26.0% and 40.5%, respectively. These results suggested that the cumulus cells recognized the PS molecules on sperm via CD36 and this molecular interaction possibly triggered the phagocytosis of sperm by cumulus cells. These results suggest that cumulus cells might play a role in inhibiting undesired immune reactions induced by sperm antigens.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalJournal of Reproductive Immunology
Volume88
Issue number1
DOIs
Publication statusPublished - 01-01-2011

Fingerprint

Cumulus Cells
Spermatozoa
Sperm Head
Phosphatidylserines
Sperm-Ovum Interactions
Annexin A5
Phagocytosis
Oocytes
Anti-Idiotypic Antibodies
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Yang, X. Z. ; Kumamoto, K. ; Hasegawa, A. ; Komori, S. ; Koyama, K. / Engulfment of spermatozoa by cumulus cells and the role of CD36. In: Journal of Reproductive Immunology. 2011 ; Vol. 88, No. 1. pp. 24-31.
@article{f9c6d7c636544b5a99a5b1c8fab9d03b,
title = "Engulfment of spermatozoa by cumulus cells and the role of CD36",
abstract = "The function of cumulus cells after sperm penetration is not well understood. The present study examined the phagocytic action of cumulus cells on sperm after dispersion of cumulus-oocyte complexes. In a co-incubation system of cauda sperm and cumulus cells, the sperm heads were beginning to vanish after 2.5 h and 77{\%} ± 1.34 of sperm heads had disappeared at 30. h. Most of the sperm heads were engulfed by cumulus cells. Immunofluorescent studies showed that cumulus cells were expressing the CD36 molecule, and sperm were exposing phosphatidylserine (PS). Anti-CD36 antibody and annexin V inhibited the engulfment of sperm by cumulus cells by 26.0{\%} and 40.5{\%}, respectively. These results suggested that the cumulus cells recognized the PS molecules on sperm via CD36 and this molecular interaction possibly triggered the phagocytosis of sperm by cumulus cells. These results suggest that cumulus cells might play a role in inhibiting undesired immune reactions induced by sperm antigens.",
author = "Yang, {X. Z.} and K. Kumamoto and A. Hasegawa and S. Komori and K. Koyama",
year = "2011",
month = "1",
day = "1",
doi = "10.1016/j.jri.2010.09.001",
language = "English",
volume = "88",
pages = "24--31",
journal = "Journal of Reproductive Immunology",
issn = "0165-0378",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Engulfment of spermatozoa by cumulus cells and the role of CD36. / Yang, X. Z.; Kumamoto, K.; Hasegawa, A.; Komori, S.; Koyama, K.

In: Journal of Reproductive Immunology, Vol. 88, No. 1, 01.01.2011, p. 24-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Engulfment of spermatozoa by cumulus cells and the role of CD36

AU - Yang, X. Z.

AU - Kumamoto, K.

AU - Hasegawa, A.

AU - Komori, S.

AU - Koyama, K.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - The function of cumulus cells after sperm penetration is not well understood. The present study examined the phagocytic action of cumulus cells on sperm after dispersion of cumulus-oocyte complexes. In a co-incubation system of cauda sperm and cumulus cells, the sperm heads were beginning to vanish after 2.5 h and 77% ± 1.34 of sperm heads had disappeared at 30. h. Most of the sperm heads were engulfed by cumulus cells. Immunofluorescent studies showed that cumulus cells were expressing the CD36 molecule, and sperm were exposing phosphatidylserine (PS). Anti-CD36 antibody and annexin V inhibited the engulfment of sperm by cumulus cells by 26.0% and 40.5%, respectively. These results suggested that the cumulus cells recognized the PS molecules on sperm via CD36 and this molecular interaction possibly triggered the phagocytosis of sperm by cumulus cells. These results suggest that cumulus cells might play a role in inhibiting undesired immune reactions induced by sperm antigens.

AB - The function of cumulus cells after sperm penetration is not well understood. The present study examined the phagocytic action of cumulus cells on sperm after dispersion of cumulus-oocyte complexes. In a co-incubation system of cauda sperm and cumulus cells, the sperm heads were beginning to vanish after 2.5 h and 77% ± 1.34 of sperm heads had disappeared at 30. h. Most of the sperm heads were engulfed by cumulus cells. Immunofluorescent studies showed that cumulus cells were expressing the CD36 molecule, and sperm were exposing phosphatidylserine (PS). Anti-CD36 antibody and annexin V inhibited the engulfment of sperm by cumulus cells by 26.0% and 40.5%, respectively. These results suggested that the cumulus cells recognized the PS molecules on sperm via CD36 and this molecular interaction possibly triggered the phagocytosis of sperm by cumulus cells. These results suggest that cumulus cells might play a role in inhibiting undesired immune reactions induced by sperm antigens.

UR - http://www.scopus.com/inward/record.url?scp=78650309414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650309414&partnerID=8YFLogxK

U2 - 10.1016/j.jri.2010.09.001

DO - 10.1016/j.jri.2010.09.001

M3 - Article

C2 - 21095012

AN - SCOPUS:78650309414

VL - 88

SP - 24

EP - 31

JO - Journal of Reproductive Immunology

JF - Journal of Reproductive Immunology

SN - 0165-0378

IS - 1

ER -