Enhanced expression of mRNAs of antisecretory factor-1, gp96, DAD1 and CDC34 in human hepatocellular carcinomas

Kenji Tanaka, Nobuo Kondoh, Masahiro Shuda, Osamu Matsubara, Nobuo Imazeki, Akihide Ryo, Toru Wakatsuki, Akiyuki Hada, Narihide Goseki, Toru Igari, Kazuo Hatsuse, Tsukasa Aihara, Sankichi Horiuchi, Naoki Yamamoto, Mikio Yamamoto

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

To identify differentially expressed genes in hepatocarcinogenesis, we performed differential display analysis using surgically resected hepatocellular carcinoma (HCC) and adjacent non-tumorous liver tissues. We identified four cDNA fragments upregulated in HCC samples, encoding antisecretory factor-1 (AF), gp96, DAD1 and CDC34. Northern blot analysis demonstrated that these mRNAs were expressed preferentially in HCCs compared with adjacent non-tumorous liver tissues or normal liver tissues from non-HCC patients. The expression of these mRNAs was increased along with the histological grading of HCC tissues. These mRNA levels were also high in three human HCC cell lines (HuH-7, HepG2 and HLF), irrespective of the growth state. We also demonstrate that sodium butyrate, an inducer of differentiation, downregulated the expression of AF and gp96 mRNAs, supporting in part our pathological observation. Immunohistochemical analysis revealed that gp96 and CDC34 proteins were preferentially accumulated in cytoplasm and nuclei of HCC cells, respectively. Overexpression of these genes could be an important manifestation of HCC phenotypes and should provide clues to understand the molecular basis of hepatocellular carcinogenesis.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1536
Issue number1
DOIs
Publication statusPublished - 30-04-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology

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