Enhanced freezability of goat spermatozoa collected into tubes containing extender supplemented with bovine serum albumin (BSA)

Hideaki Yamashiro, Haifeng Wang, Yasuhisa Yamashita, Kanako Kumamoto, Takato Terada

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5% BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition of 0.04% SDS and 4% glycerol. Sperm motion parameters were evaluated after post-thawing and during a thermal resistance test. The acrosome status of frozen-thawed spermatozoa was also observed using FITC-PNA staining. In frozen semen that was collected into tubes containing extender supplemented with 5% BSA, the post-thawed spermatozoa exhibited a significant improvement in motion parameters and maintained high motility throughout incubation and acrosome integrity, as compared with semen collected into tubes containing extender supplemented with lower concentrations of BSA. In conclusion, semen collection into tubes with a large volume of extender containing high concentrations of BSA dramatically improves the motility and acrosome integrity of frozen-thawed spermatozoa. This suggests that the in vitro functional freezability of spermatozoa is abruptly modified by reducing contact with seminal plasma and by flash contact with BSA at ejaculation.

Original languageEnglish
Pages (from-to)407-414
Number of pages8
JournalJournal of Reproduction and Development
Volume52
Issue number3
DOIs
Publication statusPublished - 07-07-2006
Externally publishedYes

Fingerprint

bovine serum albumin
goats
spermatozoa
semen
acrosome
ejaculation
seminal plasma
trehalose
egg yolk
thawing
pellets
glycerol
heat
testing

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

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title = "Enhanced freezability of goat spermatozoa collected into tubes containing extender supplemented with bovine serum albumin (BSA)",
abstract = "The purpose of this study was to investigate the effects of semen collection into tubes containing extender supplemented with BSA on the cryosurvival of goat spermatozoa. Semen was collected from two goats into empty tubes or tubes containing 10 ml extender supplemented with 0, 0.1, 1, or 5{\%} BSA, and the washed spermatozoa were frozen as pellets in egg yolk-trehalose extender with the addition of 0.04{\%} SDS and 4{\%} glycerol. Sperm motion parameters were evaluated after post-thawing and during a thermal resistance test. The acrosome status of frozen-thawed spermatozoa was also observed using FITC-PNA staining. In frozen semen that was collected into tubes containing extender supplemented with 5{\%} BSA, the post-thawed spermatozoa exhibited a significant improvement in motion parameters and maintained high motility throughout incubation and acrosome integrity, as compared with semen collected into tubes containing extender supplemented with lower concentrations of BSA. In conclusion, semen collection into tubes with a large volume of extender containing high concentrations of BSA dramatically improves the motility and acrosome integrity of frozen-thawed spermatozoa. This suggests that the in vitro functional freezability of spermatozoa is abruptly modified by reducing contact with seminal plasma and by flash contact with BSA at ejaculation.",
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Enhanced freezability of goat spermatozoa collected into tubes containing extender supplemented with bovine serum albumin (BSA). / Yamashiro, Hideaki; Wang, Haifeng; Yamashita, Yasuhisa; Kumamoto, Kanako; Terada, Takato.

In: Journal of Reproduction and Development, Vol. 52, No. 3, 07.07.2006, p. 407-414.

Research output: Contribution to journalArticle

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AU - Terada, Takato

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