Enhanced production of leukotriene B4 by peripheral blood mononuclear cells in patients with fulminant hepatitis

F. ASANO, H. MORIWAKI, Y. SHIRATORI, M. SHIMAZAKI, T. SAKAI, Y. KOSHINO, N. MURAKAMI, J. SUGIHARA, H. OHNISHI, K. SAITO, Y. MUTO

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Abstract

Abstract The production rate of leukotriene B4 (LTB4) was measured using peripheral blood mononuclear cells (PBMC) in patients with fulminant hepatitis (FH) or other liver diseases. LTB4 in the culture media of PBMC under stimulation with Ca‐ionophore was fractionated by HPLC and measured by radioimmunoassay. The production rate of LTB4 was elevated in 16 of 17 FH patients (3.3 ± 0.2 ng/106 cells for 5 min), while the production was below detectable level in patients with acute or chronic hepatitis and in healthy controls. In FH patients, the highest production rate of LTB4 was observed in the initial period of the disease. Enhanced LTB4 production may indicate the primed state of PBMC — the primed mononuclear cells are regarded as participating in the development of massive liver necrosis and of other organ failures in FH.

Original languageEnglish
Pages (from-to)228-231
Number of pages4
JournalJournal of Gastroenterology and Hepatology
Volume8
Issue number3
DOIs
Publication statusPublished - 06-1993

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All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

ASANO, F., MORIWAKI, H., SHIRATORI, Y., SHIMAZAKI, M., SAKAI, T., KOSHINO, Y., MURAKAMI, N., SUGIHARA, J., OHNISHI, H., SAITO, K., & MUTO, Y. (1993). Enhanced production of leukotriene B4 by peripheral blood mononuclear cells in patients with fulminant hepatitis. Journal of Gastroenterology and Hepatology, 8(3), 228-231. https://doi.org/10.1111/j.1440-1746.1993.tb01191.x