TY - JOUR
T1 - Enhanced production of leukotriene B4 by peripheral blood mononuclear cells in patients with fulminant hepatitis
AU - ASANO, F.
AU - MORIWAKI, H.
AU - SHIRATORI, Y.
AU - SHIMAZAKI, M.
AU - SAKAI, T.
AU - KOSHINO, Y.
AU - MURAKAMI, N.
AU - SUGIHARA, J.
AU - OHNISHI, H.
AU - SAITO, K.
AU - MUTO, Y.
PY - 1993/6
Y1 - 1993/6
N2 - Abstract The production rate of leukotriene B4 (LTB4) was measured using peripheral blood mononuclear cells (PBMC) in patients with fulminant hepatitis (FH) or other liver diseases. LTB4 in the culture media of PBMC under stimulation with Ca‐ionophore was fractionated by HPLC and measured by radioimmunoassay. The production rate of LTB4 was elevated in 16 of 17 FH patients (3.3 ± 0.2 ng/106 cells for 5 min), while the production was below detectable level in patients with acute or chronic hepatitis and in healthy controls. In FH patients, the highest production rate of LTB4 was observed in the initial period of the disease. Enhanced LTB4 production may indicate the primed state of PBMC — the primed mononuclear cells are regarded as participating in the development of massive liver necrosis and of other organ failures in FH.
AB - Abstract The production rate of leukotriene B4 (LTB4) was measured using peripheral blood mononuclear cells (PBMC) in patients with fulminant hepatitis (FH) or other liver diseases. LTB4 in the culture media of PBMC under stimulation with Ca‐ionophore was fractionated by HPLC and measured by radioimmunoassay. The production rate of LTB4 was elevated in 16 of 17 FH patients (3.3 ± 0.2 ng/106 cells for 5 min), while the production was below detectable level in patients with acute or chronic hepatitis and in healthy controls. In FH patients, the highest production rate of LTB4 was observed in the initial period of the disease. Enhanced LTB4 production may indicate the primed state of PBMC — the primed mononuclear cells are regarded as participating in the development of massive liver necrosis and of other organ failures in FH.
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U2 - 10.1111/j.1440-1746.1993.tb01191.x
DO - 10.1111/j.1440-1746.1993.tb01191.x
M3 - Article
C2 - 8390869
AN - SCOPUS:0027315689
SN - 0815-9319
VL - 8
SP - 228
EP - 231
JO - Journal of Gastroenterology and Hepatology
JF - Journal of Gastroenterology and Hepatology
IS - 3
ER -