Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells

Osamu Maeda, Masashi Kondo, Takayoshi Fujita, Noriyasu Usami, Takayuki Fukui, Kaoru Shimokata, Takafumi Ando, Hidemi Goto, Yoshitaka Sekido

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30 Citations (Scopus)

Abstract

The Hedgehog (Hh) signaling pathway and the Wnt signaling pathway are known to play important roles in carcinogenesis and the progression of various human malignant tumors. Although a relationship between these two pathways has recently been reported, the mechanism by which β-catenin, one of the key molecules of the Wnt signaling pathway, influences the Hh pathway has not yet been revealed in detail. To clarify the role of β-catenin in relation to the Hh signaling pathway, we transfected GLI1 and β-catenin expression constructs into human malignant cells, including stomach, colon, and lung cancers, and evaluated the luciferase activity of GLI-responsive reporter constructs. While exogenous GLI1 increased the luciferase activity, exogenous β-catenin also enhanced the activity under overexpression of GLI1. However, co-transfection with T-cell factor (TCF)-4 or lymphocyte enhancer factor (LEF)-1 did not influence the activity, indicating that the enhancement of β-catenin in relation to the Hh signaling pathway is not TCF/LEF-dependent. Our results suggest that β-catenin might be involved in the Hh signaling pathway via enhancement of the transcriptional activity of GLI.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalOncology reports
Volume16
Issue number1
Publication statusPublished - 01-07-2006

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Maeda, O., Kondo, M., Fujita, T., Usami, N., Fukui, T., Shimokata, K., Ando, T., Goto, H., & Sekido, Y. (2006). Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells. Oncology reports, 16(1), 91-96.