Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells

Osamu Maeda, Masashi Kondo, Takayoshi Fujita, Noriyasu Usami, Takayuki Fukui, Kaoru Shimokata, Takafumi Ando, Hidemi Goto, Yoshitaka Sekido

Research output: Contribution to journalArticle

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Abstract

The Hedgehog (Hh) signaling pathway and the Wnt signaling pathway are known to play important roles in carcinogenesis and the progression of various human malignant tumors. Although a relationship between these two pathways has recently been reported, the mechanism by which β-catenin, one of the key molecules of the Wnt signaling pathway, influences the Hh pathway has not yet been revealed in detail. To clarify the role of β-catenin in relation to the Hh signaling pathway, we transfected GLI1 and β-catenin expression constructs into human malignant cells, including stomach, colon, and lung cancers, and evaluated the luciferase activity of GLI-responsive reporter constructs. While exogenous GLI1 increased the luciferase activity, exogenous β-catenin also enhanced the activity under overexpression of GLI1. However, co-transfection with T-cell factor (TCF)-4 or lymphocyte enhancer factor (LEF)-1 did not influence the activity, indicating that the enhancement of β-catenin in relation to the Hh signaling pathway is not TCF/LEF-dependent. Our results suggest that β-catenin might be involved in the Hh signaling pathway via enhancement of the transcriptional activity of GLI.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalOncology Reports
Volume16
Issue number1
Publication statusPublished - 01-07-2006
Externally publishedYes

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Catenins
Neoplasms
Wnt Signaling Pathway
Luciferases
Transcription Factor 7-Like 2 Protein
Lymphocytes
TCF Transcription Factors
Colonic Neoplasms
Stomach Neoplasms
Transfection
Lung Neoplasms
Carcinogenesis

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Maeda, O., Kondo, M., Fujita, T., Usami, N., Fukui, T., Shimokata, K., ... Sekido, Y. (2006). Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells. Oncology Reports, 16(1), 91-96.
Maeda, Osamu ; Kondo, Masashi ; Fujita, Takayoshi ; Usami, Noriyasu ; Fukui, Takayuki ; Shimokata, Kaoru ; Ando, Takafumi ; Goto, Hidemi ; Sekido, Yoshitaka. / Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells. In: Oncology Reports. 2006 ; Vol. 16, No. 1. pp. 91-96.
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Maeda, O, Kondo, M, Fujita, T, Usami, N, Fukui, T, Shimokata, K, Ando, T, Goto, H & Sekido, Y 2006, 'Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells', Oncology Reports, vol. 16, no. 1, pp. 91-96.

Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells. / Maeda, Osamu; Kondo, Masashi; Fujita, Takayoshi; Usami, Noriyasu; Fukui, Takayuki; Shimokata, Kaoru; Ando, Takafumi; Goto, Hidemi; Sekido, Yoshitaka.

In: Oncology Reports, Vol. 16, No. 1, 01.07.2006, p. 91-96.

Research output: Contribution to journalArticle

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Maeda O, Kondo M, Fujita T, Usami N, Fukui T, Shimokata K et al. Enhancement of GLI1-transcriptional activity by β-catenin in human cancer cells. Oncology Reports. 2006 Jul 1;16(1):91-96.