TY - JOUR
T1 - Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059
AU - Murata, Takayuki
AU - Hijikata, Makoto
AU - Shimotohno, Kunitada
N1 - Funding Information:
We wish to thank Dr. Gram (Novartis Pharma, Basel) for providing CGP57380. This work was supported by grants-in-aid for cancer research and by the second-term comprehensive 10-year strategy for cancer control and by the Ministry of Health, Labor, and Welfare, as well as grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology, grants-in-aid by the Japanese Society for the Promotion of Science (JSPS) and by the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR) of Japan. T.M. is a recipient of a JSPS Postdoctoral Fellowship.
PY - 2005/9/15
Y1 - 2005/9/15
N2 - Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.
AB - Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.
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U2 - 10.1016/j.virol.2005.06.015
DO - 10.1016/j.virol.2005.06.015
M3 - Article
C2 - 16005928
AN - SCOPUS:23944482498
SN - 0042-6822
VL - 340
SP - 105
EP - 115
JO - Virology
JF - Virology
IS - 1
ER -
