Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Takayuki Murata, Makoto Hijikata, Kunitada Shimotohno

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

Original languageEnglish
Pages (from-to)105-115
Number of pages11
JournalVirology
Volume340
Issue number1
DOIs
Publication statusPublished - 15-09-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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