Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Takayuki Murata, Makoto Hijikata, Kunitada Shimotohno

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

Original languageEnglish
Pages (from-to)105-115
Number of pages11
JournalVirology
Volume340
Issue number1
DOIs
Publication statusPublished - 15-09-2005
Externally publishedYes

Fingerprint

Hepacivirus
Virus Replication
Extracellular Signal-Regulated MAP Kinases
Internal Ribosome Entry Sites
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
RNA
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

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Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059. / Murata, Takayuki; Hijikata, Makoto; Shimotohno, Kunitada.

In: Virology, Vol. 340, No. 1, 15.09.2005, p. 105-115.

Research output: Contribution to journalArticle

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