Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Takayuki Murata; Makoto Hijikata; Kunitada Shimotohno

doi:10.1016/j.virol.2005.06.015

Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Takayuki Murata
, Makoto Hijikata
, Kunitada Shimotohno

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

Original language	English
Pages (from-to)	105-115
Number of pages	11
Journal	Virology
Volume	340
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2005.06.015
Publication status	Published - 15-09-2005
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Virology

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10.1016/j.virol.2005.06.015

Cite this

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title = "Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059",

abstract = "Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.",

keywords = "ERK, HCV, IRES, Replication",

author = "Takayuki Murata and Makoto Hijikata and Kunitada Shimotohno",

note = "Funding Information: We wish to thank Dr. Gram (Novartis Pharma, Basel) for providing CGP57380. This work was supported by grants-in-aid for cancer research and by the second-term comprehensive 10-year strategy for cancer control and by the Ministry of Health, Labor, and Welfare, as well as grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology, grants-in-aid by the Japanese Society for the Promotion of Science (JSPS) and by the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR) of Japan. T.M. is a recipient of a JSPS Postdoctoral Fellowship.",

year = "2005",

month = sep,

day = "15",

doi = "10.1016/j.virol.2005.06.015",

language = "English",

volume = "340",

pages = "105--115",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

AU - Murata, Takayuki

AU - Hijikata, Makoto

AU - Shimotohno, Kunitada

N1 - Funding Information: We wish to thank Dr. Gram (Novartis Pharma, Basel) for providing CGP57380. This work was supported by grants-in-aid for cancer research and by the second-term comprehensive 10-year strategy for cancer control and by the Ministry of Health, Labor, and Welfare, as well as grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology, grants-in-aid by the Japanese Society for the Promotion of Science (JSPS) and by the Program for Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR) of Japan. T.M. is a recipient of a JSPS Postdoctoral Fellowship.

PY - 2005/9/15

Y1 - 2005/9/15

N2 - Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

AB - Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

KW - ERK

KW - HCV

KW - IRES

KW - Replication

UR - https://www.scopus.com/pages/publications/23944482498

UR - https://www.scopus.com/pages/publications/23944482498#tab=citedBy

U2 - 10.1016/j.virol.2005.06.015

DO - 10.1016/j.virol.2005.06.015

M3 - Article

C2 - 16005928

AN - SCOPUS:23944482498

SN - 0042-6822

VL - 340

SP - 105

EP - 115

JO - Virology

JF - Virology

IS - 1

ER -

Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Abstract

UN SDGs

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